Discovery of chitin synthase gene sequence in silkworm.

Chitinase gene sequences of different nuclear insects are different.

If NCBI can't find it,

Then there is no other place.

If it's not published,

Primers can be designed using existing sequences.

Cloning homologous genes of Bombyx mori by nested PCR

After obtaining the target sequence, sequencing and redesigning primers.

Full-length transgenic tobacco mutants with 3,5-terminal RACE were used for functional verification.

After this process, you can do your doctoral thesis.