Main courses: developmental biology, histology and embryology, microbiology and human health. The research fields mainly involve cell molecular biology, aquatic molecular genetics, bioinformatics and so on. At present, the research direction is: cell culture of mantle of Hyriopsis cumingii and cytology-assisted pearl culture. In this paper, two national patents and several national, provincial and school-level projects were obtained through the primary culture of Hyriopsis cumingii mantle cells and the study of pearl formation mechanism, and they were applied to pearl cultivation practice. Research topic 1. National Natural Science Foundation for Youth: Screening and Identification of Functional Genes Related to Biomineralization in Hyriopsis cumingii II. Project funded by the Ministry of Education for Young Teachers: Expression and functional analysis of functional genes of calcium metabolism in Hyriopsis cumingii. Shanghai postdoctoral fund project: molecular mechanism of calcium metabolism in Hyriopsis cumingii; 4. Open subject of key disciplines of aquatic biology: Study on the mechanism of important regulatory proteins related to calcium ion information transmission in Hyriopsis cumingii on Ca2+ metabolism; 5. Outstanding young teachers of Shanghai Education Commission supported the project: Study on the mechanism of calcium metabolism by calmodulin in Hyriopsis cumingii; 6. Doctoral research start-up fund of Shanghai Ocean University: "Construction of expression vector of calmodulin, an important regulator of calcium metabolism in Hyriopsis cumingii, and its influence on pearl formation"; 7. National Natural Science Foundation Project: Regulation of microRNA on Muscle Development of Paralichthys olivaceus during Metamorphosis: 8. Shanghai University Natural Science Research Project: Establishment of epidermal cells of mussel mantle and in vitro culture of artificial pearls; 9. Shanghai Science and Technology Commission Curriculum Construction Project: Ocean 085 Engineering Curriculum Construction Project. Li Wenjuan, Shi Zhiyi *, Hao Yingying, Jin Yuli. Determination of calcium flux in mantle of Hyriopsis cumingii by microelectrode technique. Journal of Aquatic Biology, 20 1 1, 35 (3): 544-549. Li Wenjuan, Shi Zhiyi * Hao Yingying, Ye Xianfeng. The accumulation and distribution of Ca2+ in Hyriopsis cumingii tissues were studied by laser focusing microscope. Journal of fisheries of china, China, 20 1 1, 35 (2): 2 14-2 19. Hao Yingying, Shi Zhiyi *, Li Wenjuan and Jin Yanli. The effect of VD3 on Ca2+ concentration in mantle cells of Hyriopsis cumingii was observed by laser focusing microscope. Journal of Zoology, 20 1 1, 46 (3): 98- 103. Jin Yanli, Shi Zhiyi *, Li Wenjuan, Hao Yingying, Qiang Gang. Triangle. 2011,20 (5): 705-711.Shi Zhiyi *, Hao Yingying, Li Wenjuan, Han Jian, Jin Yanli. Effects of three different factors on Ca2+ fluidity of mantle cells of Hyriopsis cumingii, China Journal of Cell Biology, 2065438. Jin Yuli. Cloning and protein purification of Nacrein gene from Hyriopsis cumingii and its effect on pearl crystal formation, Biotechnology Bulletin, 20 10,12:137-141. He Xiujuan and Shi Wenjuan. Effects of nuclear transfer on three immune defense factors in haemolymph of Hyriopsis cumingii. Patents and awards: national patent: cultivation method of large-scale nucleated pearls. National patent: double-head nuclear feeder for pearl culture. Excellent Practice Tutor of Shanghai Ocean University (20 12) School-level Excellent Course of Developmental Biology of Shanghai Ocean University (20 12) First Prize of Excellent Academic Papers of Shanghai Fisheries Society (20 10).