1: Insert the copper hook into the spinal cord of a dead frog and hang it on the iron railing. Frogs twitch when their legs touch the iron bars.

2. Put the frog in a copper dissecting dish. When the scalpel touches the frog's leg, it will twitch.

According to his experiments, he concluded that the "bioelectricity" in the frog's own muscles and nerves was the cause of convulsions. In 2008+079 1 year, Gavagni published a paper on bioelectricity in muscle, which attracted wide attention.