Genetic experiments in universities cover classical genetics, cytogenetics and other fields, including confirmatory experiments, which enable students to gradually deepen their understanding of genetic knowledge from individual morphology, cell level to chromosome level, as well as comprehensive experiments and applied experiments.
For example, the genetics experiment course of Sun Yat-sen University contains 9 experimental items, including genetics experiments of animals, plants and humans. ?
Model animal Drosophila melanogaster is the main experimental material in the genetics experiment course of Nanjing University. Through the operation of classical experiments of genetics, we should strengthen our understanding of the three laws of genetics and master the important basic concepts of genetics. At the same time, comprehensive experiments at molecular level such as reverse genetics and epigenetics have been added.
What steps does the scientific hybridization experiment of gene experiment include?
Steps of Drosophila single factor scientific hybridization experiment
1. Selection of virgin flies: each group 1 bottle, each group 1 bottle, orthogonal selection of wild red eye as female parent and mutant white eye as male parent, all fruit flies in the old bottle of female parent were killed or released under anesthesia, 5 virgin flies were collected within 8- 12h, and the virgin flies and 5 male flies were transferred to a new hybrid.
2, 7 days later, release the hybrid parents (must be clean);
After 3.4-5 days, adult flies with F 1 appeared. After 7 days of killing their parents, the eye expression and sex phenotype of F 1 were observed and recorded.
4. Five pairs of F 1 generation Drosophila melanogaster were selected, transferred to new culture bottles and cultured at 25℃ to kill the remaining F 1 generation Drosophila melanogaster;
Five or seven days later, F 1' s parents were killed.
6. After another 5 days, the F2 adult appeared, and began to observe and record the winking and sex, counting for 7 days.