Calculation formula of freezing point drop: △ TF = KF * B.
0.543= 1.68*b
B = 0.29mol mol/kg
If the mass of the solvent is 1kg, the amount of glucose is 0.29mol and the mass is m = nm = 0.29 *180 = 52.2 g.
Mass fraction = 52.2/(1000+52.2) *100% = 5%.
Osmotic pressure calculation formula: π=cRT, c≈b of dilute solution.
π= cRT = 0.29 * 8.3 14 *(273. 15+37)= 747.8 pa = 0.7478 kpa
Note: The absolute zero of the above T is -273. 15℃, or -273℃, and the calculated result is 747.4Pa.