Find the gene sequence of human insulin, synthesize it artificially and connect it to the plasmid vector of yeast, which should have the marker gene. Culture and screen the yeast successfully connected, and then culture and test whether its product is insulin. Yeast whose product is insulin is selected for culture, and the produced insulin is collected, separated and purified, and verified in experiments and clinical applications.

Firstly, human insulin gene is cloned-connecting yeast vector to construct yeast expression vector-recombinant is transferred into yeast cells-a large number of yeast cells containing insulin can be obtained by 30-degree culture-breaking yeast cells, extracting insulin-purifying insulin protein to obtain a large number of insulin.

Extended data:

Biochemistry and biophysics use chemical and physical methods to study biological problems at different levels, such as molecular level, cell level, whole level and even group level. Molecular biology focuses on studying the universal laws of life activities at the molecular level (including multi-molecular systems);

② At the molecular level, molecular biology focuses on macromolecules, mainly protein, nucleic acids, lipid systems and some polysaccharides and their complex systems. The transformation of some small molecular substances in organisms belongs to the category of biochemistry;

Baidu Encyclopedia-Molecular Biology