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The First Affiliated Hospital of Henan University

nucleic acid testing

The collection time is from 9: 00 am to 12: 00 am from Monday to Sunday.

The First Affiliated Hospital of Henan University is located at No.357, Ximen Street, Longting District, Kaifeng City, and the gathering place is in the infectious disease clinic of the First Affiliated Hospital of Henan University. You can call 037 1-22736935 if you want to consult.

Principle of novel coronavirus nucleic acid detection

The most common method for detecting novel coronavirus-specific sequences is fluorescence quantitative PCR. Because the template of PCR reaction is only DNA, novel coronavirus nucleic acid should be reverse transcribed into DNA before PCR reaction. In the PCR reaction system, there are a pair of specific primers and a Taqman probe. The Taqman probe is a specific oligonucleotide sequence, and both ends of the probe are marked with a reporter fluorescence group and a quenching fluorescence group respectively. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quenching group; If the target sequence exists in the reaction system, the probe will bind to the template during the PCR reaction, and DNA polymerase will digest the probe along the template by using the nucleic acid exonuclease activity of the enzyme, and the reporter group will be separated from the quenching group to emit fluorescence. Every time a DNA strand is amplified, a fluorescent molecule is produced. The fluorescence quantitative PCR instrument can monitor that the number of cycles of fluorescence reaching the preset threshold is related to the concentration of viral nucleic acid. The higher the concentration of viral nucleic acid, the smaller the Ct value. The products of different manufacturers will determine the positive judgment value of this product according to the performance of their own products.