Professional introduction
Based on years of teaching in biochemistry, bioinformatics, genetic engineering, fermentation engineering and molecular biology, biochemistry and molecular biology majors also include biochemical drugs, genetically engineered drugs, immunology, interaction between plants and microorganisms, and transgenic stress-resistant plants. The growing point is to study and clarify the biochemical metabolic process of organisms by means of molecular biology and reveal the mechanism of their metabolic changes. Focus on the separation, purification, enrichment, structural identification, transformation or creation of bioactive substances such as drugs, enzymes, antibiotics, toxins, etc., explore the methods and molecular mechanisms of immunotherapy for invertebrates and important crops, so as to stimulate and enhance their potential disease resistance, environmental pollution resistance and drought resistance, predict and confirm the structure and function of some special macromolecular substances, and clarify the molecular mechanisms in the evolution of animals, especially insects, so as to vigorously promote related disciplines, especially
Training objectives
Cultivate basic theories, knowledge and skills of biological science and receive good professional skills training; It has a good potential to continue studying for master's and doctoral degrees, and at the same time has scientific and technological talents who use the theoretical knowledge and skills they have mastered.
research direction
At present, there are four stable research directions in biochemistry and molecular biology, namely:
1, biochemical and bioengineering drugs
Using advanced biochemistry and genetic engineering technology, research functional drugs with the potential to prevent and treat human diseases, including biochemical separation technology, separating and purifying effective components with medicinal functions such as enzymes, protein, peptides, polysaccharides and glycoproteins from animals, plants and microorganisms, and studying their biochemical properties and pharmacological activities, especially the functions of thrombolysis, anti-radiation, anti-inflammation, anti-aging and immune antibodies; The functional protein gene was cloned into prokaryotic or eukaryotic expression system by gene recombination technology, and the engineering strain was constructed to obtain the target genetic engineering drug. It mainly includes two aspects:
(1) Obtaining bioactive drugs Use advanced biotechnology to efficiently separate, purify or prepare bioactive drugs closely related to human health (such as fibrinolytic enzyme for dissolving thrombus, polysaccharide for reducing blood lipid, low molecular weight chitosan for inhibiting cancer, etc.). ), and constantly improve the miniaturization and refinement of separation, purification and identification methods, and clarify the nature, composition, structure, related genes and protein sequence of bioactive drugs, and obtain them through gene cloning or site-directed mutation. (2) The discovery and identification of tumor markers, using the advanced method of protein's genomics, through the reaction characteristics between tumor markers and serum of cancer patients, realize the early diagnosis of cancer.
2, molecular immunology:
The purpose of this study is to establish a method or technical system to make animals and plants gain immunity or high resistance to biotic and abiotic stresses such as diseases, poisons, drought, salinity, low temperature, etc., to clarify the molecular mechanism of their immune resistance, to explore the signal molecules and their modes of action in the process of immune response, and to research and develop immune preparations and immune vaccines in order to achieve the purpose of popularization and utilization; In addition, related resistance functional genes are obtained by molecular biology technology and introduced into target animals or plants for expression, so as to obtain new varieties with enhanced immunity or high resistance. It mainly includes two aspects:
(1) Animal molecular immunity: Taking Musca domestica and Fenneropenaeus China as objects, the innate immune response mechanism of animals in the process of resisting pathogens was studied, including the function of antibacterial factors, the signal pathway of their production and release and the regulation process; (2) Immune-induced plant disease resistance: With potato, strawberry and cotton as the main targets, the method, mechanism and practical application effect of pre-inducing plants or transferring exogenous disease resistance genes with active substances of animals, plants or microorganisms and inducing their expression were studied.
3. Molecular genetics and behavior
This research direction is mainly based on DNA homologous recombination and gene knockout technology, and reveals the molecular genetic mechanism of animal behaviors such as smell, reproduction, obesity, learning and memory from different levels and multiple levels such as animal behavior, neuroanatomy, cell, biochemistry and molecule.
4. Genetic diversity and molecular evolution
This research direction mainly studies the molecular mechanism and adaptive evolution of insect system evolution. According to the molecular evolution information of insect genetic material inside and outside the nucleus, including the information contained in the complete sequence of mt genome, the complete sequence of nuclear 18S rDNA, the complete sequence of 28S rDNA and the sequence of functional gene Hox, as well as the macroscopic morphological results, the systematic evolution of ORTHOPTERA and the phylogenetic relationship among various groups were discussed.
Course introduction
Advanced biochemistry
Reveal the composition, structure and movement law of living matter at the molecular level; It is the basic knowledge needed by all disciplines in the field of modern biological sciences. The content of this course mainly includes the following parts: (1) glycoconjugates (2) protein (the basic component of protein structure; The hierarchy of protein structure, the determination of protein structure, the degradation of protein, the folding of protein, etc. (3) Enzymes (4) Biofilm and signal transduction. At the same time, the latest research progress in the field of biochemistry is introduced in combination with the latest progress, covering dynamic and cutting-edge knowledge.
molecular biology
This course first introduces the meaning of molecular biology, its position in life science, its development status and prospect, and the structure, replication, transcription, translation, regulation, mutation, repair and recombination of DNA. At the same time, taking into account the development trend of the discipline, this paper focuses on the application technologies of molecular biology, such as molecular cloning tools, electrophoresis technology, vectors, DNA and RNA preparation, DNA library construction, genetic transformation, gene expression, PCR, and also introduces the synthesis and analysis of protein. It aims to make graduate students understand the new progress of modern molecular biology theory and provide knowledge and technology for related disciplines to clarify problems at the molecular level.
Comprehensive experiment of modern biology
This course focuses on cultivating students' comprehensive experimental ability to apply biological (especially biochemical and molecular biology) experimental means and engage in biological experiments. This course welcomes students to combine their own research direction, select relevant materials, and engage in the experiment of this course purposefully, but requires students to contact the teacher one semester in advance in order to make corresponding preparations and arrangements. The content includes two parts, namely, genetic engineering part and protein part: the acquisition of gene sequence and the design of PCR primers; PCR gene amplification technology; Preparation of competent cells of Escherichia coli; Transformation of foreign genes by calcium chloride method; Alkali cracking to extract a small amount of plasmid; Positive clones were identified by enzyme digestion; IPTG-induced target protein expression; Separation and purification of target protein; The relative molecular weight of protein was determined by SDS-PAGE. Identification of target protein by protein blot; Objective To detect protein by ELISA.
Special topics in biological sciences
This course teaches the latest research progress in biochemistry and molecular biology, such as glycobiology, nucleic acid chemistry, protein structure and function, genetic engineering, protein engineering and fermentation engineering. At the same time, students are required to learn the latest research literature, discuss and write progress reports, so that students can master the development trend of this discipline and do a good job in scientific research.
Biostatistics and software applications
Biostatistics is an interdisciplinary subject between biology and mathematical statistics. Using mathematical statistics to study and solve biological problems is one of the important means of modern biological research. This course mainly introduces the basic principles and methods of biostatistics, involving basic statistical analysis methods such as hypothesis testing, variance analysis, nonparametric testing, regression and correlation analysis. , and using the method of operating on the computer as the main method, introducing data analysis software to draw charts and statistical analysis of commonly used experimental or survey data. Help students discover laws and hidden information from a large number of data. Master the basic application of commonly used data analysis software.
bioinformatics
Bioinformatics is to extract, store, process and analyze all kinds of biological information (especially molecular biological information) by using advanced data management technology, analysis model and calculation software, which provides a powerful tool for exploring complex life phenomena and their laws. Courses for graduate students include: the development trend of bioinformatics and its research contents and methods; Biological information network resources and common search tools; Double sequence alignment; Nucleic acid and protein database.