2. Taking natural products as the leading structure, introducing fluorine, heterocyclic ring, α-aminophosphonate and 2- cyanoacrylate compounds into the structure to create green pesticides. The anti-TMV activity and anti-cancer activity of cyanoacrylate compounds were reported for the first time in the world, and various new green synthesis methods were studied through microwave and ultrasonic radiation, which laid the foundation for further designing new antibacterial molecules.
3. A series of new environmental protection fungicides were developed to control the main soil-borne diseases of rice, cotton, vegetables, fruits and tobacco. A new solvent-based closed-loop synthesis process of hymexazol powder was developed for the first time, and the synthesis method, process conditions, analysis methods, quality control and development of new preparation of Guangkuling were integrated and innovated. Independently design and build an industrial production device with an annual output of 100 tons of hymexazol powder and 1000 tons of guangkuling preparation; The yield of hymexazol powder is higher than that of similar products at home and abroad by more than 10%. Two new catalysts were developed for the first time to synthesize intermediates and methyl chloropicrin, respectively, which improved the reaction efficiency and reduced the formation of by-products. The original three procedures of separation, neutralization and extraction in the old process are omitted, which greatly shortens the process flow and production cycle; Take the lead in realizing the industrial production of 95% methyl chlorophosphate powder and preparation in China; High-purity raw powder of methyl phoxim was developed, which was registered by the Ministry of Agriculture for the first time and produced exclusively in China, and developed into the leading pesticide for the prevention and treatment of soil-borne diseases in China, which promoted the upgrading of old pesticides with high toxicity and high residue for the prevention and treatment of soil-borne diseases in China, and promoted the progress of pesticide industry and the development of plant protection in China.
4. Study on bioenergy production and comprehensive utilization of resources of characteristic plants in karst mountainous areas;
Focusing on China's energy strategy, according to the resource situation and provincial conditions of Guizhou Province, the biodiesel production and comprehensive utilization of tung oil, a characteristic plant species in karst mountainous areas, were studied. Major milestones include:
(1) Investigation of Jatropha curcas Resources and Screening of High-quality Provenances
The resource situation of Jatropha curcas in southwest China was investigated, and the natural distribution area and suitable planting area of Jatropha curcas were determined, especially the site selection of Jatropha curcas afforestation. Soxhlet extraction method was determined as the first choice for seed oil extraction laboratory and as the standard method for evaluating the oil content of Jatropha curcas. The hot pressing method was determined as the extraction method of seed oil in industrial production, and the technological conditions of oil extraction were optimized. On this basis, the oil content of Jatropha curcas seeds in Guizhou, Sichuan, Yunnan and other regions was analyzed and compared, and the fatty acid composition of seed oil in each region was studied. Combined with the evaluation of main economic and technical indexes such as seed yield and oil content, the origin of high-quality provenance of Jatropha curcas was determined.
(2) Study on the technology of producing biodiesel from tung oil.
The processes of producing biodiesel by liquid phase method and solid phase catalysis method have been studied successively, and the development of high-efficiency and high-activity solid catalyst has been completed. On this basis, the process of producing biodiesel by solid-phase catalysis was completed. The conversion rate is 99%, and the yield of methyl ester is over 95%. The whole transesterification process (including post-treatment) does not produce three wastes, which is a clean production process. A 300-ton/year pilot production line was built, and the pilot test was completed, achieving good results. The analysis method of fatty acid methyl ester in tung oil biodiesel was studied, and the gas chromatography analysis method was established. The sample of biodiesel produced is about100t, and all indicators of the sample are better than domestic zero diesel, and the emission reaches the Euro IV standard. Guizhou Zhongshui Energy Development Co., Ltd. adopted the new technical achievements of solid-phase catalysis developed by our team, built and put into production 1 10,000 tons Jatropha curcas biodiesel production line, and realized industrialization.
(3) The physical and chemical indexes of biodiesel were tested and its performance was evaluated.
The biodiesel samples were sent to Guangdong Petroleum Product Quality Supervision and Inspection Center to detect the physical and chemical indexes of biodiesel products. In cooperation with four German companies, such as DaimlerChrysler, Volkswagen, Shell and Bosch, 65438±0.20kg biodiesel samples were provided to Germany, and the physical and chemical indexes and comprehensive performance evaluation tests of biodiesel were carried out. The results show that the physical and chemical indexes of tung oil biodiesel meet the current European biodiesel standards EN 65 438+04, 265 and 438+04. The National Professional Laboratory of Automotive Power and Emission Measurement of Beijing Institute of Technology conducted a comprehensive emission test (CO, HC, NOX and particulate matter) on diesel engine power, economy, full-load smoke and exhaust pollution. The results show that Jatropha curcas biodiesel is equivalent to 0# diesel engine in power and economy, and its emission performance is significantly better than 0# diesel engine. The National Quality Supervision and Inspection Center of Chongqing Heavy Duty Truck has tested the power, economy and emission of the whole vehicle. The results showed that Jatropha curcas biodiesel was equivalent to 0# diesel.
(4) Study on the comprehensive utilization of Jatropha curcas resources.
Extraction, separation, purification, structural identification and anticancer activity screening were carried out on the residue (oil cake after crude oil extraction), leaves, stems, skins, roots and other parts of Erythrina seed, and the natural active component GU 170 was screened out for 5 & micro； At the concentration of m, the inhibitory activity on the proliferation of prostate cancer PC3 cells reached 8 1. 1%. In addition, diterpenoid compound 0528 1 in10&:micro; The proliferation inhibition rates of PC3, breast cancer cell BCap-37 and human gastric cancer cell BGC823 were 82.8%, 79.7% and 75% respectively. Through in-depth research and development of these active ingredients, it is possible to develop new anti-tumor drugs.
In the expert appraisal organized by the Science and Technology Department of Guizhou Province, the expert group agreed that the above work has reached the leading level of similar research in China.