The ecosystem of innovation and entrepreneurship education in Chinese studies is a new concept to describe entrepreneurship education. Entrepreneurship education ecosystem is a self-organized, dynamic and interdependent organic whole, which is permeated with the innovation and inheritance of entrepreneurship knowledge flow, and is composed of education curriculum, students, teachers, school entrepreneurship education environment and social entrepreneurship environment. Taking the entrepreneurship education ecosystem as the investigation object, the course content, teaching methods, learning tools, classroom satisfaction and school entrepreneurship education environment were investigated for the first time.