It is not true that heterotrophic bacteria use inorganic carbon sources in shrimp culture technology. The working principle of biological flocculation technology is to balance the C:N ratio (carbon-nitrogen ratio) in the pond by adding carbon sources such as molasses and sugar, so as to stimulate the growth of heterotrophic bacteria and form biological flocculation particles. Biological floc is a collection of organic substances suspended in water, including unicellular algae, multicellular algae, feces, feed residues, dead microorganisms, bacteria and invertebrates.