Transcriptome is widely used in abiotic stress research. Although there are differences in different stress mechanisms, the idea of using transcriptome technology for research is basically similar. The key is to explore the regulation mechanism of resistance difference.
Title: Transcription level analysis reveals the changes of gene regulation and signal transformation pathway in Cai Wu under temperature stress.
Publication: BMC genomics
Release date: September, 20021year
Research contents: By setting low temperature, high temperature and control group, the response mechanism of color rape to temperature was explored.
Results:
(1) According to the transcriptomics study, compared with the control group, the differentially expressed genes of HT and LT were 10702 and 7267 respectively.
(2) In order to further study the key genes of color rape temperature response. GO and KEGG annotations of differential genes showed that photosynthesis and photosynthetic antenna protein pathway were very important in the temperature response mechanism of color rapeseed. In addition, it was further found that the alleviation of high temperature greatly restricted the expression of important genes in photosynthesis pathway, while low temperature would lead to the increase of the expression of some key genes in this pathway. To sum up, colorful seedlings showed better photosynthetic performance at low temperature than at high temperature.
According to the above results, it is speculated that plants can obtain higher cold tolerance by up-regulating the expression of photosynthetic genes under low temperature stress. On the contrary, high temperature stress inhibited the expression of key genes and weakened the self-regulation ability of plants.
Key words: drought stress; Rice; Reproductive period; Variety specificity; Transcript accumulation
Publication: Physiological Plants
Release date: 202 1 1 month
Research contents: The tissues (leaves, flowers and roots) of N22 (drought-tolerant) and IR64 (drought-sensitive) plants at heading stage after drought treatment were sequenced and compared.
Results:
(1) It was found that the number of differentially expressed genes in N22 was almost twice that of IR64. Many differentially expressed genes are located in QTL related to drought. These QTLs are related to grain yield and drought tolerance, as well as drought tolerance and key plant traits related to drought.
(2) The * * * expression analysis of differential genes revealed several key genes known to be involved in drought stress. At the same time, it was found that the 1366 differential gene showed completely opposite regulatory patterns in two rice varieties under similar drought conditions.
(3) These species-specific differential genes interact with 1300 genes. Among them, 32 genes interacted with other species-specific differential genes. The promoter regions of these genes also have sequence differences between the two rice varieties. This shows that the difference of transcription regulation is of great significance to the formation of drought tolerance in plants. Sequence-based variation (promoter) can partially explain the unique variety-specific transcription behavior.
Key words: salt stress; Rice seeds; Transcriptional changes; Seed quality.
Publication: Frontier Plant Science
Publication date: 202165438+February
Research content: To explore the changes of rice seed quality under salt stress. Compared with ordinary land planting, the accumulation of sodium, magnesium, potassium and other minerals in the seeds of japonica rice planted in high-salt areas increased greatly, the yield decreased, the heading was delayed and the grain weight decreased. Therefore, RNA-seq technology was used to analyze the transcriptome of high-salt and normal-developing seeds.
Results:
The enrichment analysis of (1) GO shows that the up-regulated genes are closely related to the metabolism and stress response of biomolecules such as amino acids, lignin, polysaccharides and chitin.
(2) Through the analysis of metabolic pathways, the up-regulated genes are involved in the biosynthetic pathways of abscisic acid and melatonin, and the relationship between trehalose, raffinose and maltose and ecological stress.
(3) Transcription factors up-regulated by developing seeds under salt stress include bHLH, MYB and heat shock protein.
These can be used as potential targets for seed quality regulation under salt stress. The purpose of this study is to provide useful reference for clarifying the relationship between seed response mechanism and seed quality decline under salt stress, and to provide potential strategies for seed quality improvement under salt stress.
Title: The analysis of physiology and transcriptomics reveals a new insight into the culture-specific response of alfalfa to alkaline stress. )
Publication: Eco-toxicity and Environmental Safety
Release date: 202 1 1 1.
Research contents: The physiological and transcriptomics analysis of two alfalfa varieties with different sensitivity to alkaline conditions was carried out. After alkali treatment, the chlorophyll content and aboveground fresh weight of alkali-sensitive variety Algonquin (AG) decreased sharply, while the alkali-tolerant variety Gongnong 1(GN) maintained a relatively stable growth and chlorophyll content.
Results:
The physiological analysis of (1) shows that the Ca/Mg ion content of GN is higher than that of AG. Under alkaline conditions, the ratio of ca/Mg/Na ion, proline and soluble sugar, and the activities of peroxidase (POD) and catalase (CAT) decreased.
(2) Transcriptome analysis identified three genes differentially expressed by alkali reaction between the two varieties; Forty-eight genes were induced in two varieties (CAR), of which 574 genes were from tolerant varieties (TAR) and 493 genes were from sensitive varieties (SAR).
(3) GO and KEGG analysis showed that CAR gene was mainly involved in phenylpropane biosynthesis, lipid metabolism and DNA replication and repair. TAR gene is rich in metabolic pathway, secondary metabolites synthesis, MAPK signaling pathway, flavonoids and amino acid biosynthesis; SAR gene is especially rich in vitamin B6 metabolism.
This study provides a new idea for the study of alkali tolerance mechanism of alfalfa.
In order to resist environmental stress, plants have evolved interrelated regulatory approaches so that they can respond and adapt to the environment in time. Abiotic stress conditions affect many aspects of plant physiology and cause extensive changes in cell processes. The study of plant resistance mechanism after abiotic stress is of great significance for subsequent breeding. The study of stress is often carried out in the form of constructing "adversity" environment, looking for "different people" and exploring "different changes" Usually, the study of plant stress resistance is based on the difference of physiological indexes, and then the transcriptome technology is used to find the key nodes or genes of stress resistance at the transcription regulation level for subsequent research.
References:
1. Yuan, Lingyun, etc. "Transcription profiles reveal changes in gene regulation and signal transduction pathways under temperature stress." * BMC genomics * Volume 22, 1 687. September 22nd, 202 1, doi:10.186/s12864-021-07981-9.
2. Variety-specific transcript accumulation in rice reproductive stage under drought stress. Plant Physiology,10.111/ppl.13585. 15 202 1，doi: 10. 1 1 1 1/ppl . 13585
3. The transcription changes of developing rice seeds under salt stress suggest the goal of manipulating seed quality. Frontiers of Plant Science Volume 12 748273. 20265438 1 1 8th of the month
4. Physiological and transcriptomics analysis revealed a new insight into the variety-specific response of alfalfa to alkali stress. Ecotoxicology and Environmental Safety, Volume 228 1 130 17. 202 1，doi: 10. 10 16
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