Chinese name: Lin Shuangjun Nationality: China Occupation: Former Research Assistant, School of Pharmacy, University of Wisconsin-Madison, USA Graduate School: Resume, research direction, published papers and resume of Shanghai Institute of Pharmacy, China Academy of Sciences. In July 2002, he graduated from Institute of Chemistry, Chinese Academy of Sciences, engaged in biotransformation and biocatalysis research, and obtained a doctorate in organic chemistry. 65438+From February 2002 to August 2005, he was engaged in postdoctoral research in the Department of Chemistry, university of alberta, Canada, mainly engaged in biochemical characterization and application of glycosyltransferase in oligosaccharide synthesis and chemical synthesis of oligosaccharides. In September 2005, she worked as a research assistant in the School of Pharmacy, University of Wisconsin-Madison, USA, mainly engaged in the biosynthesis of acetylene antibiotics. He returned to China in September 2008 and was hired as a special researcher by Shanghai Jiaotong University. Now he works in the Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiaotong University. * * * Published 2 1 SCI research papers, including 3 JACS, 2 PNAS, Nature Structural &；; There are 1 articles in molecular biology and 2 articles in JOC, a professional journal of organic chemistry. Research direction: 1. Biosynthesis and binding of secondary metabolites of antimicrobial and antitumor microorganisms. Microbial secondary metabolites are natural products produced by microorganisms, which usually have complex structures and many active functional groups. The synthesis of these natural products is usually based on small molecules and assembled through a series of continuous enzyme-catalyzed reactions. By cloning the biosynthetic gene cluster responsible for the target natural products, the biosynthetic pathway of natural products in organisms and the mechanism of enzyme-catalyzed reaction are clarified. Then, the synthetic genes or regulatory genes in the synthetic pathway of the target natural products are reasonably modified to produce "unnatural" natural products with similar structures and enrich the material basis for screening physiologically active compounds. 2. Screening and identification of natural products with physiological activities from microorganisms Since the discovery of penicillin in the late 1920s, microorganisms are still an important source of drugs and drug lead compounds. Based on the high probability that microorganisms in extreme environment (including deep-sea microorganisms, thermophilic microorganisms, halophilic microorganisms, barotropic microorganisms and microorganisms of biological origin) produce compounds with novel structures, the metabolites of microorganisms are screened, separated, purified and identified with major diseases such as tumors, cardiovascular diseases, diabetes and infectious diseases as targets. 3. As an important biocatalyst, enzyme has excellent chemical selectivity, regional selectivity and stereoselectivity. On the basis of understanding the catalytic mechanism of enzymes that catalyze the biosynthesis of natural products, the application of enzyme catalysis in organic synthesis is expanded and the research of organic synthesis methodology is enriched. Main representative papers published: 1. Linsj, Van Lanen SG and Shen B *, "An independent condensation enzyme that catalyzes the formation of ester bonds in C- 1027 biosynthesis". Go on. Naturally. Akkad Sci。 USA 2009,106,4183-4188. 2.Lin SJ, Van Lanen SG and Shen B*, "Characterization of a two-component FAD-dependent monooxygenase SgcC, which requires a substrate bound by a carrier protein for the biosynthesis of an alkyne antitumor antibiotic C- 1 027". J. Am。 Chemistry. Socialists 2008,130,6616-6623.3. Regional specific chlorination of Lin Shijun, Fan Lanen, Shen Bo * ,( S)-? -Tyryl -S- carrier protein catalyzed by SgcC3 in the biosynthesis of the antitumor antibiotic C- 1027. J. Am。 Chemistry. Socialists 2007,129,12432-8.4. Van Lanen SG, Lin SJ and Shen B*, "The biosynthesis of diendiyne antitumor antibiotic C- 1027 involves a new branching point in chloride metabolism * * * ”, Proc. Nature. Akkad Sci。 USA 2008,105,494-499. (In C & ampe News, 2008 1 month 14, p36) 5. Luo Yi, Lin Shijun, Zhang Jun, Cooke HA, Bruner SD and Shen B*, "Region-specific O- methylation of naphthoic acid catalyzed by NcsB 1, which is an O- methyltransferase, participated in the biosynthesis of neoplastatin, an anticancer antibiotic of diendiyne". J. Biol。 Chemistry. 2008, 283,14694-14702.6. Van Lanen SG, Lin SJ, Dorrestein PC, Kelleher NL, Shen B*, "Adenylase SGCC/kloc involved in the biosynthesis of diene antitumor antibiotic C- 1027. 2006, 28 1, 29633-29640.7.Rose NL，Completo GC，Lin SJ，McNeil M，Palcic MM*，Lowary TL*。 Expression, purification and characterization of galactofuran glycosyltransferase involved in arabinogalactan biosynthesis of Mycobacterium tuberculosis. Chemistry. Socialists 2006,128,6721-6729.8. greco A, He JGS, Lin SJ, Palcic MM, Rupnik M, Ng KKS*, "Identification of Clostridium difficile toxin A in carbohydrates" and its natural structure. Molecular Biology 2006, 13, 460-46 1. 9.Ma B, Audette GF, Lin SJ, Palcic MM, Hazes B and Taylor DE*, "Purification, kiic Characterization and Mapping of the Minimal Catalytic Domain and Key Polar Groups of Helicobacter Pylori α-( 1, 3/ 1, 4)- fucosyltransferase" J. Biol. Chemistry. 2006, 281,6385-6395.10. Lin Shijun, Tan Chao, Jiang Sheng, Li, Judy *, "C9 iridoids in Radix Scrophulariae in Northeast China" Journal of Traditional Chinese Medicine 2006, 89, 2789-2793. 1 1. Bai Yan, Lin shijie, qi, Palcic MM and Lovari TL*, "n-octyl 2,6-dideoxy -α-L- phenoxy-hexopyranosyl -( 1-2)-3- amino -3- deoxy-? -D- galactose-pyranoside, a carbohydrate of H- disaccharide antigen *** og "Study 2006,341,1702- 1707. 12.Ridgway KM, Shi W, Lin SJ, Palcic MM and Lowary TL*, "chemical and chemical enzymatic synthesis of triterpenoid glycoside fragments from Tsukamurella pauromebola lipo arabinomannan". Canadian Journal of Chemistry 2006,84,642-649. 13. Wang Xiaoming *, Lin Shijie, Liu Jun and Zheng. "Efficient biocatalytic synthesis of high enantiomeric purity? Alkylated arylglycine and amide. Catalysis 2004,346,439-445. 14. Wang Mingxia *, Lin Shijun, Liu Chunsheng, Zheng He and Li Jiansheng, "Biotransformation of Nitriles for Efficient and Enantioselective Synthesis of Electrophilic Ethylene Oxide Carboxamides". Chemistry. 2003, 68, 4570-4573.15. Wang MX * and Lin SJ "Practical and convenient enzymatic synthesis of enantiomerically pure α -amino acids and amides". Chemistry. 2002, June 7, 6542-6545.16. Wang MX * and Lin SJ, "Efficient and enantioselective synthesis of L- arylglycine and D- arylglycine amide from nitrile biotransformation". Tetrahedral letters 2 00 1, 42, 6925-6927. 17. Guo Li, Lin Shijun, Yang, Qi Li, Wang Mingxia, Chen Yan *, "Rapid resolution of aryl glycine amides by high performance capillary electrophoresis-? -Cyclodextrin as a chiral selector ". Journal of chromatography A2003,998 (1-2): 221-228. 18. Guo Li, Lin Shijun, Dai Desheng, Yang, Qi Li, Wang Mingxia, Chen Yuying *, "Enantiomeric separation? -Quaternary arylglycine amide and human serum albumin by capillary electrophoresis "Analysis Express 2003,36 (7):145/-1462. 19. Lin Shijie, Jiang Shaohui, Li, Zeng, Judy *, "Two new iridoid compounds in Scrophularia ningpoensis" Quadruple newsletter 2000,41,1069- 107 1. 20. dinger t, Wee S, malvi GL, Greco A, kitovan, sun j, Lin SJ, klassen JS, Palcic MM, Ng KK, Armstrong GD*, "functional characteristics of o toxin expressed by Clostridium difficile, carboxyl terminal host cell binding domains of TcdA and TcdB". Glycobiology 2008,18,698-706. 2 1. Jiang Sheng, Wang Hongqiong, Li, Lin Shijun, Tan, Zhu Dilei. "two new c 18- norditerpenoid alkaloids from Aconitum flavum", China chemical newsletter, 2007, 18,409-41.