1. First, dissolve the substance to be separated in an appropriate solvent to ensure that the concentration of the sample is within the range that can be detected by the detector. At the same time, we should pay attention to avoid impurities or other interference in the sample, choose the appropriate chromatographic column, and carry out washing and balancing operations as required to ensure the performance and separation effect of the chromatographic column.

2. Secondly, appropriate chromatographic conditions such as mobile phase, flow rate, column temperature, etc. are selected and optimized as needed to obtain the best separation effect. The detector records and analyzes the detection signal to obtain the high performance liquid chromatogram.

3. Finally, according to the peak shape, peak height, peak area and other parameters on the chromatogram, make qualitative or quantitative analysis and draw corresponding conclusions.