White spot syndrome virus (white

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symptomes complice

Virus (WSSV) is the most important pathogen that harms shrimp culture, with strong pathogenicity and wide spread, which has caused great losses to shrimp culture in the world. However, there is still no effective control method. In order to effectively control diseases, it is necessary to understand the immune mechanism of shrimp. In this paper, firstly, the RNAi technology for studying the gene function of shrimp virus was established by WSSV, and then the role of QM protein in the immune process of shrimp was studied, which provided scientific basis for establishing effective prevention and control methods of shrimp diseases.

Using RNAi method, vp28 gene (the main membrane protein encoding WSSV) which plays an important role in WSSV infection was selected as the interference target gene. After synthesizing siRNA in vitro, the interference experiment was carried out by injection in vitro. The results showed that sequence-specific siRNA interference inhibited the transcription and expression of vp28 gene. At this time, competitive quantitative PCR detection showed that the interference of vp28 significantly reduced the copy number of virus particles in shrimp and delayed the death of shrimp. Therefore, WSSV established RNAi method for gene function research. After repeated injection of siRNA for three times, WSSV in shrimp was completely eliminated, which indicated that RNAi had antiviral effect in shrimp, and provided a new and effective method for the prevention and treatment of white spot syndrome virus (WSSV) in shrimp.

The prawn QM gene (PjQM) was obtained by RACE. The sequence analysis showed that PjQM had the basic characteristics and functional domains of QM protein. The fusion protein was obtained by prokaryotic expression and purification of the gene. RT-PCR and Western

Western blot analysis showed that PjQM was transcribed and expressed in all tissues of shrimp. real time

PCR analysis showed that the expression of PjQM gene was up-regulated after being stimulated by virus, which indicated that PjQM was involved in the immune process of shrimp. Through GST pull-down analysis and mass spectrometry identification, it was found that there was an interaction between PjQM protein and hemocyanin and myosin. Further experiments in vitro and in vivo proved that there was indeed an interaction between PjQM and hemocyanin. Protein interaction suggests that PjQM may participate in shrimp immunity through phenol oxidase activation system. The function of PjQM was studied by RNAi technology established in WSSV gene function research. upper

Imprinting and protein

The results of blot showed that the transcription and expression of PjQM gene were inhibited by PjQM sequence-specific siRNA, and the activity of phenol oxidase (PO) in shrimp was significantly reduced. Therefore, the results of RNAi showed that QM could regulate the activity of phenol oxidase (PO) in shrimp. The above results indicate that one of the possible ways for QM protein to participate in antiviral immunity is that QM regulates the activity of PO through its interaction with hemocyanin. This is the first time that QM has been found to play a role in animal immune response.