PAGE purification: PAGE purification is a method of separating DNA fragments by denaturing polyacrylamide gel electrophoresis, and then recovering the target DNA from the gel. PAGE purification is also a very effective method for DNA purification. The purity of purified DNA is above 95%, which is harmful to long-chain oligonucleotides.

Deoxyribonucleic acid

(greater than 50mer) is particularly effective.

HPLC purification: HPLC purification uses the principle of high performance liquid chromatography to purify DNA fragments. The purity can be greater than 99%. Mainly used for the purification of short-chain and modified primers. The weakness of this method is high cost and low mass production efficiency.

protein

G

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The biology gang has a detailed introduction.