1, edited by Zhizhong Cui, Color Atlas of Diagnosis and Treatment of Poultry Diseases, China Agricultural Press, 2003,No. 1 Edition 2. Zhizhong Cui, editor-in-chief, Veterinary Immunology (undergraduate textbook), China Agricultural Publishing House, the first edition in 2004. This textbook won the National Excellent Textbook Award of Agricultural Universities in 2005. 3. Zhizhong Cui, editor-in-chief, Guide to Veterinary Immunology Experiment (undergraduate textbook), China Agricultural Publishing House, first edition in 2006.
Main research interests: animal molecular virology, poultry tumor diseases and immunosuppressive diseases.
Major scientific and technological achievements and achievements:
1. Since the mid-1980s, the tumor-associated 38KD phosphoprotein (pp38) of Marek's disease virus (Marek's disease virus) in chicken has been systematically and innovatively studied, and it has been at the international forefront level for many years: the pp38 gene was identified for the first time in the world (Cui et al., 1990). And completed the whole genome DNA sequencing and the analysis of the whole gene structure and regulatory components (Cui et al., 199 1) (related papers were cited 85 times in the world), and took the lead in expressing the complete pp38 gene in insect cells (,1992). According to the immune response of anti-recombinant pp38 mice serum, it is inferred (Cui et al., 1992) that there are also analogues of pp38 in non-pathogenic type II and III, which has always been considered as tumorigenic type I in academic circles. It has been cited and proved in foreign countries, and it was found and proved for the first time through experiments that the recombinant pp38 gene product can inhibit the immune response of chicks to some antigens (Cui et al., 1996 et al., 1997). Recently, based on the analysis of the DNA differences of pp38 gene between eight virulent strains and CVI988 strains, the nucleotide and amino acid sequences that lead to different antigenic determinants among different virulent or tumorigenic strains were identified. On this basis, CVI988 site-directed mutant virus was constructed, which can express virulent determinant in pp38 gene, and further proved the immunosuppression of pp38 strain and its related functional regions in chicken experiments (Zhizhong Cui et al., 1997, 1999). Therefore, it is speculated that the role of pp38 in the pathogenesis of MDV may be related to the inhibition of immune monitoring of tumor cells in chickens. This is the first MDV site-directed mutant virus related to the biological characteristics of protein in the world. Therefore, Professor Cornell University, an international authority on Marek's disease research, recently published a summary paper on the tumor caused by MDV, and when talking about the immune monitoring mechanism of MDV tumor cells escaping from chickens, he used a whole paragraph to comment on the above progress made by the candidate. He said: The work of Cui Shi and others (1996, 1997) revealed the protein of MDV coding. They found that injecting recombinant pp38 protein into chicken muscle could significantly weaken the immune response of chicken to mouse red blood cells, but did not affect the immune response to Escherichia coli. In addition, they also inserted the pp38 gene of GA virulent strain into CVI988 strain of vaccine virus, and constructed a recombinant virus strain of MDV. The level of anti-MDV antibody produced by chickens inoculated with the recombinant virus was lower than that of chickens inoculated with the original CVI988 strain virus. These results should stimulate further research in this field. (Calnek, 1998, Avian Pathology, 27: S54-S64). The molecular biological study of MDV pp38 gene and its products is of great significance to clarify the mechanism of viral tumor and the prevention of comparative medicine. Since/kloc-0 returned to China in early 1990, he has successively presided over five national natural science foundation projects (including 1 key projects) in the field of MDV molecular biology. In addition to the long-term systematic study of pp38 gene, he also identified the 79kd protein gene of MDV group-like antigen for the first time in the world, and proved and directly observed the recognition and adhesion of MDV envelope glycoprotein B to MDV susceptible cells through experiments for the first time. The upper part is 95. In MDV Molecular Biology, more than 20 papers have been published at home and abroad. 2. A monoclonal antibody immunodiagnostic reagent for avian reticuloendotheliosis virus (REV, a retrovirus) was developed and patented in the United States. Establishment of the world's first monoclonal antibody against REV isotype and subtype (Cui et al., 1986). Using this set of monoclonal antibodies, Chen et al. (1987) successfully divided REV virus strains isolated from different birds around the world into three types. Using this group of monoclonal antibodies, an enzyme-linked immunosorbent assay (ELISA) system was established, which can directly detect the antigen of REV virus from various disease samples. This method is not only highly specific, but also 50 times more sensitive than the international standard method (Cui et al., 1988). 1992, this set of ELISA reagents made of monoclonal antibodies was patented by the United States Patent Office (patent number: 5 130232). The second inventor, the first author of related papers, is the actual person who completed the research. 3. At home and abroad, the pathological nature and differential diagnosis basis of a new difficult disease of donkey & lipomatosis gravidarum were first determined. The disease is a new disease in northern China since 1960s, with an incidence rate of 10% and a mortality rate of nearly 40-50%. Because there are no characteristic clinical symptoms, this disease is called not eating before delivery. According to the pathological tissue, blood and urine biochemical research, the candidate suggested that the disease was a metabolic disease caused by disorder of glucose and lipid metabolism, and its main characteristics were increased serum total fat and systemic steatosis, especially liver steatosis. The physical properties of milky serum can be used to distinguish this disease from other diseases. The therapeutic effect of high dose glucose proves that this degeneration is reversible. 1974 published two systematic research papers in the newsletter of Hebei Institute of Animal Husbandry and Veterinary Medicine. After 1975, the Northwest Cooperation Group organized by the state also published papers one after another, all of which reported and confirmed similar results. This research achievement won the Science and Technology Achievement Award of Hebei Science and Technology Conference from 65438 to 0978, and it was the only winner.