Since then, cell identification has become a concern of scientists, and since then, more and more cell pollution incidents have begun to appear. People have begun to realize the problem of cell pollution, and the causes and preventive measures of cell cross-contamination have been announced. However, the problem has been increasing, and researchers continue to publish data obtained from misrecognized and cross-contaminated cell lines.
It is estimated that nearly 20% of the cells in foreign laboratories have the above problems, and 15-25% of the research papers lead to wrong conclusions because they use wrongly identified and cross-contaminated cells. This has caused a lot of waste of research funds and produced a lot of invalid or wrong data.
In 2008, Winand Dinjens, a molecular biologist at the Josephine Nefkens Institute, and his colleagues found that esophageal adenocarcinoma cells actually have similar genotypes to esophageal squamous cell cancer cells. These results urge them to pay attention to the problem of cell identity. Ding Jens began to verify these cell lines and compare them with the original tissues. It is found that these cell lines have different genotypes from the original tissues. In 13 esophageal adenocarcinoma cell line, three cell lines: SEG- 1, BIC- 1 and SK-GT-5 were contaminated, and these cell lines were mixed with other cancer cells. These cell lines have been applied in two clinical trials by many laboratories, and more than 100 SCI papers have been published, and American patent 1 1 item has been applied. This research result was published in the latest issue of Journal of National Cancer Institute (JNCI).
In 2007, NIH issued a notice strongly recommending authentication when using cultured cells. At the end of 2008, experts suggested that ATCC devote itself to the identification of human cell lines and formulate standard procedures for identification.
In recent years, a large number of studies have shown that STR genotyping is one of the most effective and accurate methods for cell cross-contamination and property identification, and the application of STR genotyping in cell identification has been strongly recommended by ATCC and other institutions. The data of STR typing provided by American ATCC cell bank, German DSMZ cell bank and Japanese JCRB cell bank were compared.
Suzhou genetic testing biotechnology, a domestic professional appraisal institution, is located in Suzhou Industrial Park, Jiangsu Province. It can be mailed to cell lines for identification, and it started very early. The English report provided can be directly used for submission.