1. Normal cells are not toxic, but some reagents are toxic. For example, it is inevitable to freeze cells in cell culture, and DMSO (dimethyl sulfoxide, freezing protection solution) should be added in this process. The reagent itself is toxic and belongs to slight toxicity. The oral LD50 of rats is 18g/kg. But it is permeable to human skin and irritating to eyes. But good protection, such as gloves and masks, will not have any effect. Secondly, cell culture is often used to determine the toxic effects of certain chemicals on cells. At this time, the toxicity of chemicals used for testing may have an impact on people, and protective measures need to be taken.

Second, we should pay attention to cells or cancer cells infected by virus during the culture process, especially human cells, especially when there is a wound on the body. So gloves are very important.

Therefore, when doing cell culture, you should wear gloves, masks, goggles and protective clothing to protect yourself and avoid cell pollution.