Ethidium bromide (EB) has strong mutagenicity and carcinogenicity, and it accumulates in human body for a long time. It can cause chromosome variation diseases (such as cell canceration) by inserting DNA molecules, so it is recommended to use disposable gloves for operation. Or suggest that your tutor change a fluorescent dye whose main component is anthocyanin, but the price will generally be ten times higher.
There will inevitably be some contact in the experiment, such as glue recycling and southern hybridization. I did a lot of experiments during my master's degree, and now I am in good health. There must be a lot of such experiments to be done in my doctoral thesis in the future, and there is no need to panic if I touch it occasionally.
Just like the ancient heroes in Greek legend, they flew too high and too close to the sun, and the wax on their wings was scorched and fell to death alive. Some truths can only be obtained at a corresponding price. You should be aware of this line. Many molecules, biochemical reagent, are highly toxic. I will probably put a few in the back, hoping to pay attention to protection in the experiment.
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1.DEPC (diethyl pyrocarbonate): It smells sweet and fragrant, but it hurts people! A powerful protein denaturant and suspected to be a carcinogen. Keep the bottle away from you when opening the bottle. Internal pressure will cause splashing. Wear appropriate gloves and work clothes when operating, and do it in a chemical fume hood.
2.PMSF (benzyl sulfonyl fluoride): The boss said it was neurotoxic! ! ! It is a highly toxic cholinesterase inhibitor. It is very destructive to respiratory mucosa, eyes and skin. Inhalation, swallowing or skin absorption can be fatal. Wear suitable gloves and safety glasses and always use them in chemical fume hoods. In case of contact, immediately wash glasses or skin with plenty of water and discard contaminated work clothes.
3. Acetonitrile is volatile, flammable, irritating and chemically suffocating, and it is far away from heat sources and fire sources in the fume hood.
4. Actinomycin D, a teratogen and carcinogen, is operated in a fume hood.
5. α -amanita toxin cyclic peptide is very toxic and may be fatal.
6.NN- methylene bisacrylamide is toxic and will affect the central nervous system. Don't inhale powder.
7. Toxic methanol can cause blindness.
8. Acetic acid (concentrated): It may be injured by inhalation or skin absorption. Wear gloves and goggles, preferably in a chemical fume hood.
9. Ammonium persulfate: harmful to mucous membrane and upper respiratory tract, eyes and skin, and fatal if inhaled. Wear gloves and goggles when operating. Be sure to operate in a fume hood.
10. Cesium chloride: Inhalation, swallowing or skin absorption will be harmful to health. Wear gloves and goggles when operating.
DTT: Very strong reducing agent, giving off an unpleasant smell. Inhalation, swallowing or skin absorption may be harmful to health. When using solid or high concentration storage liquid, wear gloves and goggles and operate in a fume hood.
1 1. Formaldehyde: toxic, volatile, and a carcinogen, easily absorbed through the skin, which can irritate and damage eyes, mucous membranes and upper respiratory tract. Avoid evaporation of aerosol. Wear gloves and goggles. Be sure to operate in a fume hood. Keep away from heat sources, sparks and open flames.
12.TRIzol is irritating to eyes and corrosive to skin. Once I accidentally spilled a small drop on my face, it immediately became red, and it hurt after a while, and it was fine after a week. If spilled, flush with plenty of water immediately.
In the laboratory, most of the chemical reagents you come into contact with are harmful, such as ultraviolet rays, noise pollution if the centrifuge is not good, vortex will cause finger vibration, etc., so everyone should pay attention to protect themselves and don't relax their attention to safety because of saving trouble or other reasons. In addition, pay attention to standard operation, especially those engaged in microorganisms, and don't be infected in the laboratory.
13. Sodium azide, toxic, blocks cytochrome electron transfer system.
14. Sodium fluoride, toxic and fatal, is operated in a fume hood.
15. gloves, goggles and work clothes should be worn for radioactive substances (isotope labels), and it is best to buy a set (if commercialized).
16β- mercaptoethanol may be fatal and harmful to respiratory tract, skin and eyes.
17. Methotrexate, carcinogenic and teratogenic
18. Formaldehyde is toxic, volatile, carcinogenic, irritating to eyes and respiratory tract, and far away from heat sources and fire sources in the fume hood.
19. Diacrylamide, nerve agent, effect accumulation
20. Sodium dimethyl arsonate is carcinogenic and toxic.
2 1. streptomycin, carcinogenic, causing allergic reactions.
22. Piperidine, toxic, has effects on eyes and respiratory tract, and is far away from heat sources and fire sources.
23. Putrescine, flammable and corrosive, away from heat sources and fire sources.
24. Nickel sulfate is carcinogenic and can cause genetic damage.
25. Hydrazine, toxic, explosive, away from heat and fire sources.