During DNA replication, some short DNA chains of the offspring following the chain formation are called Okazaki fragments (⑵ Connecting Okazaki fragments: Under the catalysis of DNA ligase, Okazaki fragments are connected to form a complete DNA long chain. Automatic termination: the template DNA chain has a connecting region rich in GC and AT near the transcription termination point, so that RNA can be transcribed. ......

There is Okazaki's own experimental scheme for reference:

During 1968, Reishi Okazaki designed two groups of experiments, one of which was pilse-labeling.

Experiment). Okazaki infected E.coli (t-) strain with T4 bacteriophage, and used Cscl dTTP(3H-T) for 2', 7', 15', 30', 60', 120' pulse labeling, respectively, then isolated and extracted T4 bacteriophage DNA, and denatured. The results showed that almost all newly synthesized DNA fragments were 10-20s after 3H-T labeling for different time, that is, the size was 1000-2000 nucleotides. The second group of experiments is the pulse tracking experiment.

Experiment), in order to study the developmental outcome of the 10-20s-20s fragment found in the pulse labeling experiment during the whole replication process, Okazaki first cultured the isotopically labeled experimental strain for 30 seconds, then transferred it to the normal culture medium for several minutes, separated the DNA, and sent it to ∑? I don't follow the rules? Reflect the sky? A large fragment of 0- 120s. Therefore, this replication mode of DNA is called discontinuous replication mode, and the first synthesized 10-20s-20s fragment is called Okazaki fragment.

References:

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