The rapid development of China's economy and society has accelerated the diversification of college students' values. However, the rapid development of social networks and the rise of network culture are undoubtedly of great significance to the formation of college students' values. Values are the core content in the ideological, cultural and ideological system of a country and society. Especially for college students who are in the critical period of their lives, whether they have correct values will directly affect their healthy growth and future development, the correct establishment of world outlook and outlook on life, and the stable development of the whole society. Firstly, this paper systematically analyzes the connotation of network culture through theoretical analysis, and expounds it in detail from three aspects: material level, behavior level and spiritual level. According to the structure of network culture, starting from the three-dimensional space composed of technology, cultural spirit and subjectivity, this paper distinguishes the characteristics of network culture from the new characteristics of network culture in colleges and universities, so as to make the understanding of network culture characteristics more clear, clear and prominent. Secondly, in order to have a more comprehensive understanding of college students' values in this era and better carry out targeted scientific and reasonable values education, this paper adopts the method of empirical analysis, conducts a special survey on college students' values in the form of questionnaire survey, and analyzes the new trends, new contents and new characteristics of college students' values in the new historical period from the perspectives of politics, ideals, career choice, marriage and love. Finally, using the method of combining problem discussion with countermeasure research, this paper expounds the problems existing in college students' values education under the background of network culture and the reasons for the problems. This paper puts forward the countermeasures of college students' values education in the new period: we must keep up with the background of network culture, update our concepts and establish a brand-new concept of values education in network culture; We must give full play to the characteristics of the network and establish a new mode of values education in colleges and universities based on values education and taking values education as the medium.