The utility model discloses a negative pressure dust removal chamber, which sequentially comprises an operation chamber, a transition chamber and a sedimentation chamber from left to right, wherein a grid bottom plate is arranged at the lower part of the operation chamber, and a water pool is arranged below the grid bottom plate; A grid-shaped bottom plate is also arranged at one side of the lower part of the transition chamber near the operation chamber, a water pool is also arranged below the grid-shaped bottom plate, and a fan for pumping air from the transition chamber to the settling chamber is arranged at the joint of the transition chamber and the settling chamber; A settling tank is arranged in the settling chamber, the inside of the settling tank is communicated with the outside, and a water pump is arranged in the settling tank; The horizontal height of the fan is lower than that of the top of the sedimentation tank. The utility model overcomes the shortcomings of the existing dust removal device, such as high noise, poor dust removal effect and difficult dust treatment, and provides a negative pressure dust removal chamber with low noise, good dust removal effect and simple dust treatment.