A large number of Trichoderma gene sequences have been cloned by different methods, including differential hybridization, probes synthesized based on protein sequence, probes designed by heterologous genes or primers designed and synthesized according to homologous sequences for PCR amplification. By February 25th, 20 13, in the NCBI GenBank database, * * * 23,027 records were retrieved by T-search ... The scaffold sequence of genome sequencing was screened out, and the gene sequence related to Trichoderma taxonomy and phylogenetic research was the largest number of remaining records. Including rDNA(5.8S, 18S, 28S), ITS, translation elongation factor, calmodulin, actin, tubulin, RNA polymerase II subunit, ATP citrate lyase (ATP CITY). Among other 1500 gene records with clear functions, the number of chitinase coding genes is the largest, with 6 14, including endochitinase 42 (ech42) and chitinase 18-5 (chitinase 18-5, Chi/kloc-). Chitinase 18- 13 gene (chitinase18-655chi18-15), chitinase 18- 17 (chitinase/. There are also 55 glucanase genes, such as β- 1, 4- endoglucanase (Endo- 1, 4-β- glucanase), β- 1, 3- endoglucanase, β- 1, 6- endoglucanase (endo-glucanase). There are 50 glyceraldehyde-3-phosphate dehydrogenase (gpd) genes; There are 49 cellobiohydrolase (cbh) genes; There are 30 protein kinase genes, including mitogen-activated protein kinase (Tmk) and serine/threonine protein kinase (serine/threonine protein kinase). 25 hydrophobin genes; There are 20 kinds of xylanases and related genes, including β- 1, 4- endoxylanase (Endo- 1, 4-β- xylanase) and xylanase regulators. Protease gene16; There are 12 genes that may be related to the formation of secondary metabolites, including non-ribosomal peptide synthase gene and polyketide synthase gene. There are 12 glucosaminidase, including N- acetyl -β-D glucosaminidase and exoβ-D- D-glucosaminidase. Glucosidase gene13; Expansin gene12; There are 12 nitrate reductase (euknr) genes; Six phytase genes; Laccase gene 5, etc.

conclusion

The acquisition of Trichoderma genome sequencing information has greatly accelerated the research progress of Trichoderma. These genetic material bases determine the viability and defects of different Trichoderma, thus determining their different lifestyles. Genome sequence information provides impetus for new research direction. For example, a large number of small molecular secretory proteins, new cell wall degrading enzymes and those supposed secondary metabolite biosynthetic enzymes are all new research directions, which need to be studied in genetics, chemistry, physiology and other fields. The acquisition of multiple Trichoderma genome sequences will also contribute to the exchange of research results between different species. For example, by comparing the regulation patterns of cellulase transcription factors and signal components between Trichoderma reesei and Trichoderma biocontrol, it will provide meaningful new discoveries for understanding the physiological mechanism of Trichoderma different lifestyles. By analyzing the Qualcomm genome, transcriptome and metabolic pathway of wild-type and mutant strains, new genes related to biomass degradation, biological control and human pathogenicity will be identified. The direction of future efforts will be to integrate these physiological processes and molecular maps. But new problems will appear again, which will eventually push us to leave the computer and go to the experimental platform to verify the correctness of many assumptions.