Current location - Education and Training Encyclopedia - Graduation thesis - Is it normal for liquid chromatography to have a downward curve? If this appears in the graduation thesis, will there be any problems?
Is it normal for liquid chromatography to have a downward curve? If this appears in the graduation thesis, will there be any problems?
Obviously, there is a problem. Any analysis and determination method must be "selective", which is called "specificity". The current method can't separate solvent peaks, and the selectivity is very poor, which is definitely abnormal. In addition, there is an impurity peak before the solvent peak, which means that your running time is too short, the previous sample has not finished yet, or the method itself has serious defects. As an exploratory study of analytical methods, pre-experiment is possible. If the experimental data obtained by this method is definitely not suitable for use in the paper, it means that your measurement peak has not returned to the baseline of the chromatographic system and has no selectivity. The measurement results must be inaccurate, and whether the conclusion is reliable or not is uncertain. If this map appears in your paper, the quality of the paper must be very poor.