There are two links in the rapid propagation of axillary buds of healthy seedlings: one is to form virus-free seedling materials. After the stem seeds were treated with warm water at 52℃ for 30 minutes, most viruses and pathogens parasitic on sugarcane buds were killed. Combined with high temperature germination culture at 38 ~ 40℃, on the one hand, sugarcane buds grow rapidly, on the other hand, the growth and spread of viruses and germs are inhibited, and the shoot apex meristem is farther away from the germs, which is beneficial to the removal of the germ-free shoot apex meristem, thus achieving the goal of complete detoxification and disease removal. The second is tissue culture and rapid propagation. Under aseptic conditions, sugarcane axillary buds (lateral buds) were inoculated into MS or N medium with different kinds and concentrations of plant hormones, and liquid static culture was carried out at 25 ~ 30℃ in natural scattered light environment, including axillary bud treatment, cluster bud (seedling) induction, subculture and rooting culture.