There are 5 1 universities in Nanjing, including 34 undergraduate universities (including 8 private universities) and 7 junior colleges (including 5 private universities). The better universities are Nanjing University, Southeast University, Nanjing University of Aeronautics and Astronautics, Nanjing University of Science and Technology, Hohai University, China Pharmaceutical University, Nanjing Normal University and Nanjing Agricultural University.

At present, there are 13 universities in Nanjing, all of which are "double-class" universities, among which Nanjing University and Southeast University are the two most famous universities in the region, both of which are 985 famous universities. Li Nan University, Heather University, Hohai University, Southern Agricultural University, China Southern Airlines University and China Pharmaceutical University are mostly former 2 1 1 universities, all of which have Class A disciplines, which are relatively strong among many former 2 1 1 universities in China.

Although the five universities, Nanyi, Lin Nan, Nanzhong, Nanyou and Nanxin University, have not entered the 2 1 1 project, they all have excellent disciplines, and they are all selected as "world-class discipline construction" universities in the "double-first-class" construction, and they are also quite powerful.

Nanyi, in particular, is a famous A+ medical school in China. Its medical strength ranks around 15 in domestic universities, and the admission scores of many majors are relatively high. In 2022, the enrollment major in Zhejiang Province will be 17. Although the lowest score is only 604 (nursing), the 13 major has reached 633 and above, and the score of stomatology (5+3 integration) has even reached 662.