Carbon dioxide Sugar-free tissue culture and rapid propagation technology was invented by Toyuki Ozawa, a professor at Chiba University in Japan, in the late 1980s. It is a brand-new plant tissue culture technology and an organic combination of environmental control technology and tissue culture technology. It uses co2 instead of sugar as the carbon source of plants, and adjusts the air, light, temperature, humidity and other influencing factors in tissue culture microenvironment through "one-step" technology to promote plant photosynthesis, so that tissue culture plants can be transformed from autotrophic, thus promoting.