Undergraduate students: general plant pathology, plant protection research technology, virus and human.

Postgraduates: plant virology (bilingual teaching), professional English, advanced plant pathology.

Doctoral student: Molecular Plant Virology

Research field and direction

1, Identification and Control of Plant Viral Diseases

By means of biology, serology and molecular biology, the serious and emerging virus diseases in production were quickly diagnosed and identified, and their epidemic regularity and control measures were studied to provide technical support for agricultural safety production. The virus diseases of watermelon (WMV, ZYMV, CMV, TMV and CGMMV), corn (SCMV, RBSDV and RBSDV2), tobacco (PVY, PVX, TVBMV), peanut (PStV and CMV) and tomato (yellow leaf curl, TYLCV) were studied.

The prevention and treatment measures of viral diseases are discussed from the following two aspects:

(1) antiviral genetic engineering. Transgenic tobacco plants expressing PVY, CMV and TMV simultaneously were obtained by RNAi technology. RNAi is cultivating transgenic maize resistant to dwarf mosaic disease and rough dwarf disease.

(2) Using PGPR strain to control virus diseases. Among them, PGPR has achieved good results in the prevention and treatment of tobacco virus diseases.

2. Plant virus evolution, gene function and pathogenesis.

By analyzing the viral genome sequence, the emergence and prevalence of new strains were detected, and the evolutionary mechanism of the virus was studied. It is proved that there are five strains of potato Y virus (PVY) in tobacco in China, and the existence of O:N strain is found for the first time in the world, and the occurrence of NTN and N:O strain in China is reported for the first time. By comparing HC-Pro, P3, 6K 1 and CP genes of 40 tobacco vein mosaic virus isolates, the population structure of China tobacco vein mosaic virus was analyzed, and it was proved that recombination, negative selection and gene flow were the main driving forces for the evolution of China tobacco vein mosaic virus. It is proved that the Basal-BR family of Turnip Mosaic Virus (Tumv) is in an outbreak state in China. The population structure of TuMV in China is different from that in Japan, and it has its own unique sub-population.

The role and mechanism of virus gene and its coding protein in the process of virus infection, replication, migration, aphid transmission and pathogenicity were studied by viral infectious cloning and site-directed mutation analysis. It is hoped that on the basis of clarifying the pathogenic mechanism of virus, a weak strain can be obtained for cross-protection of virus diseases.

In cooperation with Liu Huanting, a researcher at the University of St Andrews in the United Kingdom, the crystal structure of virus protein and its interacting host protein was analyzed by structural biology method, and the molecular mechanism of virus pathogenicity and host susceptibility was analyzed.

3. Phytoplasma diseases

Using molecular biology methods to diagnose phytoplasma diseases, analyze the causes and explore effective control strategies and means. The occurrence of rose witches' broom, Spiraea witches' broom, cherry greening, elm witches' broom, yellowing and Vitex negundo witches' broom in China was reported for the first time.

Undertake scientific research projects

1. Identification and structural analysis of functional complexes of tobacco vein mosaic virus HC-Pro and VPg. NSFC-RSE 20 10-20 1 1。

2. Mechanism and regulation of HC-Pro affecting the pathogenicity of tobacco vein mottle mosaic virus National Natural Science Foundation (30971895) 2010-2012.

3. Early Warning and Control Technology of Maize Rough Dwarf Disease (2009GG 100902 1) Key Science and Technology Project of Shandong Province, 20 10-20 12.

4. Breeding a New Maize Variety Resistant to Dwarf Mosaic Virus by Transgenic Technology (2009ZX080030 14B) National Major Transgenic Project 2009-201.

5. Construction and Transformation of Infectious Clone of Tobacco Vein Mosaic Virus (20080434006), Ph.D. Program Fund of Ministry of Education, 2009-201.

6. Formulation of technical standards for pathogen identification of important fruit trees Special scientific research project for quality inspection and public welfare industry (2008 105 17, cooperation project with China Academy of Inspection and Quarantine) 2009-20 1 1.

7. Study on Plant Virus Variation and Gene Function (NCET-07-0520) 2008-20 10 "New Century Excellent Talents Support Program" of the Ministry of Education.

8. Development and application of biocontrol bacteria against tobacco virus disease, 2008-201kloc-0/Shandong Tobacco Company.

9. Population Variation of Turnip Mosaic Virus (Z2007D04) 2008-20 10 Key Project of Shandong Natural Science Foundation

10.2006-2009 Occurrence regularity and control of tobacco root diseases in Shandong Tobacco Company

1 1. Transformation of aptamer to obtain multi-resistant transgenic tobacco (2004BS06002), Award Fund for Outstanding Young and Middle-aged Scientists in Shandong Province from 2005 to 2007.

12. the structure of HC-pro and its role in virus transmission, synergy and RNA silencing inhibition (3047 1 138), national natural science foundation of China, 2005-2007.

13. establishment and research of multi-functional RNA silencing inhibitor identification system (20054003), 2006 438+0-2008 438+02, special research fund for doctoral programs in institutions of higher learning of the Ministry of education.

14. Molecular Epidemiology of Tobacco Potato Virus in China, 2004.338+0-2006. 12 Swedish-Asian Cooperative Research Project (348-2003-50 1 1).

15. Study on New Strategies of Tobacco Virus Genetic Engineering "(301017), 2002+0-2004.438+02, National Natural Science Foundation of China.

Winning record

1Nomination Award of Top Ten Outstanding Youth in Shandong Province in 2002

In 2007, he was selected into the "New Century Excellent Talents Support Program".

In 2007, the occurrence regularity and control of maize dwarf mosaic disease won the third prize of scientific and technological progress in Gansu Province.

In 2009, he won the 3rd Youth Science and Technology Award of China Plant Protection Society.

He has won the teaching achievement award and teaching quality award of Shandong Agricultural University for many times.

Works and papers

He has published more than 50 papers in important academic journals at home and abroad, such as Journal of Virology, Plant Pathology, Archives of Virology, Virus Gene, Journal of Plant Pathology, Plant Diseases, China Agricultural Sciences, Journal of Plant Protection and Journal of Plant Pathology. Among them, an article in Journal of Virology has been cited for more than 40 times by Yearbook of Microbiology, Journal of Plants, Journal of Virology, Molecular Breeding and Plant Biotechnology.

14., Tian, Xu Qicong, Liu Yongguang, Gao Rui,,. Molecular detection of phytoplasma around paulownia witches' broom tree. China agricultural sciences, 20 10/0,43 (2): 304-312.

15. Cui Tingtao, Pan, Gao Rui, Shao Yunhua, Shi Chengkui, Zhu Shuifang. Molecular detection and identification of Vitex negundo phytoplasma. Forestry Science, 2009.6,45 (6):162-165.

16. Liu Jinliang, Shao Yunhua, Zhang Guangmin, Zhu Xiaoping, Yang Guangling, Shi Chengkui, Li Xiangdong. Molecular characteristics of zucchini yellow mosaic virus Shandong isolate. Journal of Plant Pathology, 2009,39 (5): 544-548.

17. Liu Yongguang, Tian,,,, Shu Huairui. Molecular identification of the pathogen of sericulture Phellinus igniarius atrophy in Shandong Province. Sericulture Science, 2009,35 (3): 463-471.

18., Zhu Qinqin, Yuan, Sun, Zhou Tao, Fan. Molecular characteristics of Shandong isolate of rice black-streaked dwarf virus causing maize rough dwarf disease. Journal of Plant Pathology, 2008,38 (5): 540-543.

19. Zhang Chengling,,, Zhang Wei, Xie,, Zhang Guangmin. Screening and identification of antagonistic actinomycetes against ginger blast. Journal of Plant Pathology, 2008,38 (4): 414-419.

20. Zhu Tiansheng, Pan, Cui Tingtao, Gao Rui, Zhu Shuifang. Molecular detection and identification of phytoplasma of elm chlorosis. Journal of Plant Pathology, 2008,38 (4): 401-406.

2 1. Gao Rui, Li Xiangdong, Wang Jie, Liu Jinliang, Zhu Xiaoping, Zhang Guangmin. Molecular identification of the pathogen of Spiraea witches' broom disease. Forestry Science, 2007+05438+0,43 (11): 72-75.

22. Blue, blue, Gao Rui, Zhu Tiansheng, Prokaryotic expression of CP gene of tobacco vein mosaic virus and preparation of antiserum, Journal of Plant Pathology, 2007,37 (5): 461-466.

23. Li Lijun, Liu Yongguang, Li Guangwei, Lan,,. Identification of burkholderia cepacia Lyc2 and its effect on preventing diseases and promoting growth of cotton seedlings. Journal of Plant Pathology, 2007,37 (4): 426-432.

24. Zhu Xiaoping, Liu Jinliang, Tian Yanping, Yu Xiaoqing, Li Xiangdong and Liu Hongmei. Transient expression and comparison of RNA silencing induced by three gene structures of CP gene of potato X virus. Journal of Applied and Environmental Biology, 2007, 13 (1): 1-4.

25. Gao Rui,, Lan,,. Cloning, prokaryotic expression and antiserum preparation of coat protein gene of cucumber mosaic virus. Journal of Plant Protection, 2007,34 (3): 335-336.

26. Zhu Changxiang, Song, Wen Fujiang,,. Resistance to potato Y virus mediated by 3'- terminal fragment of CP gene. China Agricultural Sciences, 2006,39 (6):146-1152.

27. Liu Jinliang, Yu Xiaoqing, Tian Yanping, Du Jie, Zhu Xiaoping, Li Xiangdong and Yan Dunyu. Molecular characteristics and CP gene expression of turnip mosaic virus Weifang radish isolate. Journal of Horticultural Science, 2006,33 (1): 84-88.

28. Liu Jinliang, Li Wenfa, Fan, Li Huaifang, Pathogen identification of radish heart disease in Weifang area. Journal of Plant Pathology, 2005,35 (1):13-17.

Li Xiangdong, Liu Jinliang, Li Hongkui, Shi Chengkui, Wang Honggang. Occurrence and control of radish heart disease in Weifang area. Journal of Plant Protection, 2005,32 (2): 221-222.

30. Tian Yanping, Lan, Shi Chengkui,,. Cloning and sequence analysis of 3'cDNA fragments of different turnip mosaic virus isolates. Journal of Plant Pathology, 2005,35 (6, ZK): 38-42.

34. Guo, Feng Kun, Guo Hengjun, Li Zhao. Effects of PVY/PVX synergy on virus concentration and ultrastructure of host cells. China Agricultural Sciences, 2003,36 (3): 281-286.

35. Fan, Li Huaifang, Qiu. Mechanism of wheat aphid spreading maize dwarf mosaic virus. Journal of Plant Protection, 2002,29 (1): 62-66.

36. Fan, Li Huaifang, Qiu. Purification and antiserum preparation of maize dwarf mosaic virus HC-Pro. Journal of Plant Pathology, 200 1, 31(2):135-138.

37. Fan, Li Huaifang, Qiu. Accumulation and immune localization of maize dwarf mosaic virus HC-Pro in maize leaves. Journal of Plant Pathology, 200 1, 31(4): 310-314.

38. Li Xiaodong, Li Yongqiang and Wang. Prevalence of tobacco potato Y virus and cucumber mosaic virus in Henan province. Plant diseases, 200 1, 85(4): 447

39. Li Huaifang, Fan and Qiu. Mechanism of maize dwarf mosaic virus HC-Pro in the process of aphid transmission. Journal of Plant Pathology, 2000,30 (3): 217-221.

Participated in compiling the textbook Agricultural Plant Pathology (Northern Edition) (Agricultural Press, 2002) and translating Plant Virology (Science Press, 2006).