In recent years, with the rapid development of molecular biology technology and its penetration into micronucleus research, the detection and application scope of micronucleus test has been greatly expanded, and it has developed into the detection of various genetic endpoints such as chromosome breakage, loss, delayed division, unbalanced division, gene amplification, non-separation, DNA damage repair obstacles, HPrt gene mutation, apoptosis, unbalanced cell division and so on. Therefore, in recent years, a new concept of newmi-clone test has been put forward internationally. Of course, in order to detect multiple genetic damage endpoints in an experiment, more new technical means are needed, such as FISH technology and image analysis technology, which also puts forward higher requirements for laboratory conditions and research level in China.