A good family environment made him develop the habit of reading and seeking knowledge. 65438-0926 was admitted to Peking Union Medical College, the highest medical institution at that time, with excellent results and received strict medical education. 1934 became a doctor in Peking Union Medical College Hospital after graduation. Peking Union Medical College Hospital was the best and most authoritative medical institution in China at that time, and Huang worked here for 8 years. He not only laid a solid medical foundation, but also cultivated the ability to observe, find and solve problems independently. During this period, he published an original paper on diphtheria Bacillus and its immunity, which attracted the attention of American medical journals. When he was young, Huang made many achievements in the research of cholera, streptococcus infection and plague with his keen insight and solid medical skills, and published a series of research papers. Huang's talent was highly valued by Union Medical College Hospital and was selected to study in the United States on 194 1.
During his stay in the United States, Huang pioneered a new technology of virus culture in vitro, which attracted the attention of the world virology community and laid the foundation of modern virology. At this time, the Japanese invaders are still ravaging the land of China, and the Chinese nation is at a critical juncture. He resolutely refused the repeated detention of the United States. At the end of 1943, he returned to the motherland with the heart of worrying about the country and the people and the ideal of saving the country scientifically, and served as the director of the medical team of Chongqing Central Health Laboratory. After War of Resistance against Japanese Aggression's victory, he returned to Beiping and served as the president of Beiping Branch of Central Health Laboratory.
On the eve of the liberation of Peiping, he chose to stay and wait for the birth of the new China.
After the founding of People's Republic of China (PRC), Huang's professional expertise began to be brought into play. Although there were few funds at that time and the conditions for large-scale virus research were not available, the people's government tried its best to acquire research equipment and assistants for him, and he began to study Japanese encephalitis, measles, hepatitis and other viruses. Huang is determined to contribute his intelligence to the virology in China under the leadership of China Producers' Party.
During the War to Resist US Aggression and Aid Korea, he actively responded to the call of China's * * * production party. In order to crush the enemy's germ warfare, he risked his life to go deep into the front lines of northeast China and North Korea to investigate, and made contributions to maintaining world peace with his professional skills.
Huang has been to the Soviet Union, Romania, the Netherlands, Egypt, France, the Philippines, the United States and other countries to give lectures and conduct academic exchanges. From 65438 to 0983, he led a delegation of microbiology experts from China to attend the 13th International Microbiology Conference in the United States, and was awarded the title of "Golden Key" and "Honorary Citizen" in Denton, USA.
Huang enjoys a high reputation in the world. He is a member of the editorial board of the Journal of Virology jointly organized by the Soviet Union and the socialist countries in Eastern Europe, and also serves as the editorial board of the International Journal of Virology and the American Journal of Infectious Diseases. 1983 was elected honorary member of American Infectious Diseases Society.
Huang is enthusiastic about medical virology in China. He initiated and founded Virology Society of Chinese Medical Association and Journal of Experimental and Clinical Virology (predecessor of China Journal of Experimental and Clinical Virology). He has successively edited books such as Introduction to Medical Virology, Experimental Techniques of Common Viral Diseases and Virology of China Medical Encyclopedia. During his illness and hospitalization in his later years, he also presided over the compilation of Basic and Experimental Techniques of Medical Virology and Dictionary of Medical Virology.
Huang is honest, sincere, enthusiastic, knowledgeable, rigorous and innovative. 1985 joined the China * * * production party, realizing a long-cherished wish for many years. Just as he led the researchers into a new topic with great enthusiasm-tumor research with virus immunotherapy, leukemia took his life in 1987, at the age of 77.
Virus culture technology
At the beginning of the 20th century, the international research on virus just started, and the research on virus was not mature and the methods were backward. Because virus is the smallest organism in microorganisms, it is necessary to detect the existence of virus by injecting substances containing virus into animals and observing the disease or death of animals. Obviously, this method is very primitive. Another feature of the virus is that it does not have its own enzyme system and needs to be parasitic in living cells, so the general microbial culture medium can not make the virus reproduce and survive. These two characteristics of virus make it more difficult to find a new technology for virus culture. Virus culture is the most basic and critical step in virus research. It can be said that without the establishment of new virus culture technology, there will be no breakthrough and development of virus research. Therefore, many countries have invested a lot of manpower and material resources, and many well-known scholars in the world have also explored for decades.
From 65438 to 0943, Huang published "Further Study on Titration and Neutralization of Equine Encephalitis Virus in Tissue Culture" in the United States, which immediately attracted worldwide attention and was widely recognized by peers.
This new technology can be summarized as follows:
In the first step, animal tissues are artificially digested into monolayer cells, and these cells are given certain nutrition to make them survive in test tubes.
The second step is to inoculate the virus into this cell. After a period of time, a series of pathological changes will occur in cells. Observers can indirectly judge whether the virus is reproducing by observing whether the cells are sick with a common microscope.
This new technology improves virus culture from the "animal level" of experimental animals and chicken embryos to the "cell level" of tissue culture in vitro. It is also the establishment of this technology that broadens the thinking of virologists all over the world. Virologists in many countries in the world have adopted or improved this technology, successfully found the pathogens of many viral diseases and isolated many new viruses. In 1950s, Enders, a famous American virologist, won the Nobel Prize, which was the result of using yellow technology. Huang's technology laid the foundation of modern virology, according to Who's Who in the World 1982 to 1985 published by the United States.
The practice of virology research proves that this new technology discovered by Huang plays an important role in the development of virology research to the level of molecular virology today. So far, the world has not found a more advanced method of virus culture in vitro. This new technology has been widely used in the development of vaccines for viral diseases, the production of diagnostic reagents, and high-tech research fields such as virus monoclonal antibodies and genetic engineering. Many countries in the world use this technology to isolate viruses such as epidemic hemorrhagic fever, measles and polio. In recent years, the HIV that shocked the world was also isolated through tissue culture.
Japanese encephalitis research
In the early days of the People's Republic of China, Japanese encephalitis was one of the infectious diseases that seriously threatened the health of working people. Huang clearly knows that it is very difficult to study Japanese encephalitis and solve this medical problem. However, as the first generation virologist in New China, his sense of responsibility inspired him to be a volunteer. He asked the leaders of the Ministry of Health to start virus research in New China from Japanese encephalitis. The health authorities met his wishes, supported his work and gave him the guarantee of manpower and material resources. The research work of Japanese encephalitis began.
Due to the limitation of science and technology at that time, the understanding of Japanese encephalitis was still superficial, and the causes, pathogenesis, transmission law, diagnosis and immunity of Japanese encephalitis were not solved. Even Japanese encephalitis (commonly known as encephalitis at that time) prevalent in Asian countries such as China and Japan is not clear whether it is a disease. These problems were all topics to be revealed in the field of virology at that time.
In the first two years after the founding of New China, a comprehensive and systematic investigation of the Yellow River was organized. Thanks to the strong assistance of the health department and the enthusiastic cooperation of various medical and health institutions, this work has been carried out quite smoothly. After a large number of epidemiological investigations, Huang led researchers to study the virus isolation, the establishment of experimental diagnosis methods, the vector ecology of Japanese encephalitis and the characteristics of Japanese encephalitis virus, and basically found out the epidemic law, transmission routes and characteristics of Japanese encephalitis in China, and emphatically pointed out that mosquitoes are the vector of Japanese encephalitis, thus technically guiding the vigorous patriotic health campaign in the early days of the founding of the People's Republic of China.
From 65438 to 0949, Huang first started the development of Japanese encephalitis vaccine in China. In a paper, he first expounded the idea of developing Japanese encephalitis vaccine: "When we started the study of Japanese encephalitis in 1949, we first investigated the epidemiological problems of this infectious disease, and determined that the pathogen of the disease was Japanese encephalitis virus through serology and virus separation. These research results point out the direction for prevention work. In order to better meet the needs of prevention work, we started the vaccine manufacturing experiment at 1949. " This is the earliest research record of Japanese encephalitis vaccine in China. In the following decades, the development of Japanese encephalitis vaccine has been going on, from the study of dead vaccine to the study of live attenuated Japanese encephalitis vaccine by tissue culture technology. All these research results are permeated with Huang's painstaking efforts. The development of Japanese encephalitis vaccine won the 1978 National Science Conference Award.
As we all know, the achievements of preventive medicine research institute can not be achieved by one person alone, but need the joint efforts of a long period of time, sometimes even generations. The research on JE in China began with 1949, and after 40 years of efforts, it was finally recognized by the society. 1989 this achievement won the first prize of scientific and technological progress of the Ministry of Health. At the time of awarding the prize, although Huang is no longer alive, and even his name is not on the list of winners, people will never forget Huang's pioneering position in Japanese encephalitis research in China and his great role in this achievement.
Virus immune contribution
1954, measles virus was successfully isolated in the world. Using tissue culture technology to develop measles vaccine has become an important topic in the field of virology in the world. In 196 1, Huang devoted himself to the research of measles vaccine with great enthusiasm and abundant energy. In cooperation with Professor Futang Zhu, a famous pediatrician, the pathogenicity and immunity of measles virus were deeply studied. Their cooperation promoted the research of measles virus in China at that time. Since then, Huang and his measles virus laboratory have conducted extensive research on measles virus hemagglutinin, measles vaccine adjuvant and vaccine production technology. The measles vaccine treated with formalin is an important paper he published during this period. This paper was read out at the 4th International Virology Conference and was well received by the participants.
After 1980, Huang devoted himself to the study of virus immunity, and successively published papers such as "The Influence of Passive Immunity on Active Immunity of Live Viruses". In the research of virus immunotherapy for tumors, I have guided graduate students to do exploratory work, and published several papers successively, such as "Two Preliminary Studies on Treating Ascites Mice with Different Viruses", "Study on Treating Mice Tumors with Virus and Cyclophosphamide" and "Study on Anti-macrophage Migration of Tumors". These research results undoubtedly provide valuable clues and basis for finding anti-tumor treatment methods. The new idea of tumor virus immunotherapy proposed by Huang will be a promising field in tumor treatment research. The influence of knowledge point Huang
Because of Huang's important contribution to medical virology research, he was elected as a member of the Biology Department of China Academy of Sciences on 198 1 and was appointed honorary director of the Institute of Virology, Chinese Academy of Preventive Medicine. He used to be the executive director of chinese society for microbiology, the executive director of the Society of Microbiology and Immunology of Chinese Medical Association, and the chairman of the Society of Virology of Chinese Medical Association.
After Huang's death, in order to commemorate his achievements in medical virology research, his colleagues and relatives at home and abroad initiated the establishment of the Huang Medical Virology Foundation, and awarded scholarships in Huang's name to reward newcomers who made contributions to medical virology research. The Virology Branch of the Chinese Medical Association and the Institute of Virology of the Chinese Academy of Preventive Medicine jointly edited and published Selected Papers of Huang to commemorate his outstanding contribution in virology research.