2. Genetics

3. Biochemical and Molecular Biology 1, National Natural Science Foundation Project (3 items)

(1) Study on the Interspecific Hybridization of Jute and Its Genetic Relationship (1984- 1986):20000.

(2) Study on inheritance and heterosis of kenaf long-column mutant (2001-2003): 65,438+200,000 (cooperation).

(3) Establishment of SRAP marker system and genetic linkage map of kenaf (2006-2008): 260,000.

2, the National Ministry of Science and Technology and the Provincial Science and Technology Department of agricultural scientific and technological achievements transformation award project (2)

(4) Demonstration and popularization of new kenaf varieties with high yield, high quality and stress resistance and breeding of improved varieties (Ministry of Science and Technology, 2001-2003): 300,000.

(5) Demonstration and popularization of new kenaf varieties with high yield, good quality and stress resistance and breeding of improved varieties (Provincial Science and Technology Department, 2001-2003): 300,000.

3. National 863 Plan (3 items)

(6) Kenaf efficient breeding technology and breeding of new varieties with high quality, high yield and stress resistance (20065438+0-2003): 75,000.

(7) Cultivation of new kenaf varieties (2004-2005): 50,000 plants.

(8) Rapid breeding of photo-sensitive genic male sterile lines in rice by tissue culture (1995): 88,000.

4, the national key science and technology projects (5)

(9) Study on collection, identification and utilization of kenaf germplasm resources (1980- 1985):20000.

(10) Study on the Collection, Identification and Utilization of Kenaf Germplasm Resources (1986-1990): 20,000.

(1 1) Collection, identification and utilization of kenaf germplasm resources (1991-1995): 20000.

(12) Study on the Collection, Identification and Utilization of Kenaf Germplasm Resources (1996-2000):20000.

(13) Standardization, collation, identification, breeding, storage and utilization of kenaf germplasm resources (2006-2007): 20,000.

5, the national science and technology support plan (2)

(14) Research on the Exploration and Innovative Utilization of Hemp Genetic Resources (2006-2010): 65,438+10,000.

(15) Research and preparation for efficient development and utilization of cannabis resources (2006-2008): 65,438+10,000.

6. National 948 Science and Technology Project (1)

(16) Introduction, innovation and demonstration of key technologies for hemp industrialization development (2006-2010): 500,000.

7, the national public welfare industry (agriculture) special funds for science and technology (1)

(17) Study on Efficient Production and Harvest Technology of Ramie, Yellow/Kenaf and Flax (2007-20 10): 1.45 million.

8. Fujian Natural Science Foundation (3 items)

Study on combining ability and heterosis utilization of kenaf (1995- 1997): 20000.

(19) Study on kenaf long column mutant and its heterosis utilization (2002-2004):40000.

(20) Transgenic breeding and safety evaluation of insect-resistant and herbicide-resistant kenaf (2005-2007): 40,000.

9. Key scientific research projects of Fujian Science and Technology Department (7 projects)

(2 1) Breeding, development and comprehensive utilization of new kenaf variety Fu Hong 1 and 2 (1994-1996): 20,000.

(22) Molecular Markers of Kenaf Germplasm Resources and Breeding of New Varieties for Oil and Fiber (2004-2006): 70,000.

(23) Demonstration, popularization and efficient transformation of new kenaf varieties with super high yield and high quality (2007-2008): 200,000.

(24) Study on genetic diversity and utilization of heterosis of castor germplasm resources by molecular markers (2007-2009): 65,438+10,000.

(25) Research on transgenic kenaf germplasm with insect resistance, herbicide resistance and salt tolerance (2007-2009): 65,438+10,000.

(26) Research and development of standardized rapid detection technology for hybrid rice seed purity (2003-2006): 65,438+10,000 (cooperation).

10, Science and Technology Projects of Fujian Provincial Government, Agriculture Office, Agriculture Department and Education Department (6 items)

(27) Study on breeding of new kenaf varieties with high yield and disease resistance and multi-generation utilization of their heterosis (1992-1994): 40,000.

(28) Study on Genetic Transformation of Kenaf Resistance and Its Safety Evaluation (Ministry of Education, 2007-2009): 20,000.

(29) Study on Genetic Transformation of Insect-resistant Genes into Kenaf (Provincial Plant Protection Station, 2005-2007): 25,000.

(30) Research and Development of New Healthy Edible Oil with High Linoleic Acid Kenaf and Conjugated Linoleic Acid (Governor's Fund 2006-2007): 20,000.

(3 1) Study on introduction, identification, breeding, preservation and utilization of castor germplasm resources (school fund 2006-2007): 50,000 yuan. 1. Research on collection, identification and utilization of hemp crop resources in China, 1992 won the second prize of scientific and technological progress of the Ministry of Agriculture (mainly completed);

2. Genetic relationship between yield, quality and heterosis of kenaf varieties, 1992 won the second prize of Fujian Excellent Paper (the first finisher);

3. 1997 "Research on Collection, Identification and Utilization of Hemp Germplasm Resources in China" won the third prize of National Science and Technology Progress Award (mainly completed);

4. Breeding of new kenaf variety Fu Hong No.2 with high yield and disease resistance, 200 1 won the third prize of provincial scientific and technological progress (first finisher);

5. Rice cell engineering breeding won the second prize of provincial scientific and technological progress in 2002 (mainly completed);

6. The efficient kenaf breeding technology system and the breeding of new varieties with high quality, high yield and stress resistance won the third prize of provincial scientific and technological progress in 2005 (the first finisher);

7. Research on the construction of national biological science base and talent training mode, won the first prize of provincial excellent teaching achievement in 2005 (mainly completed);

8. In June 2007, the project "Extension of New Kenaf Varieties with Super High Yield, High Quality and Disease Resistance and Breeding of Improved Varieties" participated in the Achievement Exhibition of Rural Applicable Technology and Agricultural Scientific and Technological Achievements Transformation Fund of the 4th China-ASEAN Expo, and won the Excellent Exhibition Project Award. Since 1995, nine new kenaf varieties have been bred and passed the national and provincial variety examination:

1 and new kenaf varieties Fu Hong 1, 1995 passed the variety examination in Fujian province, 1999 passed the variety examination in Anhui province, and in 2006, 5438+0 was listed as the key popularized variety for the achievement transformation of the Ministry of Science and Technology.

2. Fu Hong No.2, a new kenaf variety, was approved by Fujian Province 1995, Zhejiang Province 1997 and Anhui Province 1999, and was listed as the key variety to be popularized by the Ministry of Science and Technology in 2006.

3. Fu Hong No.3, a new kenaf variety, was approved by Fujian Province in 2004 and the Ministry of Agriculture in 2005, and was listed as the key popularization variety for the achievement transformation of the Ministry of Science and Technology in 20001year.

4. Fu Hong 95 1, a new kenaf variety, was approved by Anhui Province in 2006, Fujian Province in 2003 and the Ministry of Agriculture in 2005, and it was listed as a key variety promoted by the Ministry of Science and Technology.

5. Fu Hong 952, a new kenaf variety, was approved by Anhui Province in 20001year, Fujian Province in 2003 and Ministry of Agriculture in 2005, and was listed as the key popularization variety of achievements transformation in the Ministry of Science and Technology in 20001year.

6. Breeding of a new kenaf variety Fu Hong 99 1 approved by Fujian Province in 2004: It was approved by the Ministry of Agriculture in 2005 and listed as a key popularization variety by the Ministry of Agriculture.

7. Breeding of a new kenaf variety Guangjin 1: it passed the variety examination and approval in Fujian Province in 2004; In 2005, it was approved by the Ministry of Agriculture and listed as a key variety promoted by the Ministry of Agriculture.

8. Fu Hong 992, a new kenaf variety, passed the examination and approval of the Ministry of Agriculture in 2007, and was listed as a key variety promoted by the Ministry of Agriculture.

9. Fu Hong 99 13, a new kenaf variety, was approved by the Ministry of Agriculture in 2007, and was listed as a key variety promoted by the Ministry of Agriculture.

Fu Honghang 1, a new kenaf variety, was approved by Anhui Province in 2008.

A new kenaf variety, Fuhonghang No.2 1 1, was approved by Anhui Province in 2008.

A new kenaf variety Fuhonghang No.3 12 was approved by Anhui Province in 2008. A new kenaf variety Fu Hong 1 1 was bred, which was 7.2% higher than the control Yue 743.

2. A new kenaf variety 12 was bred, which increased the yield by 25.0% compared with the control Yue 743.

3. A new kenaf variety 13 was bred, which increased the yield by 17.8% compared with the control Yue 743.

4. A new kenaf variety 14 was bred, which increased the yield by 17.5% compared with the control Yue 743.

5. Compared with the control Fu Hong 952, the yield of Fu Hong Bt952 transgenic kenaf increased by 1 1.5%. 1. Successfully held the 2007 International Symposium on Kenaf and Homologous Fibers.

Approved by the Foreign Affairs Office of Fujian Provincial People's Government (No.64, 2007), Fujian Agriculture and Forestry University, Fujian Science and Technology Association, CCG International Company of the United States and University of Minnesota of the United States jointly sponsored the "2007 International Symposium on Kenaf and Homologous Fibers", which was undertaken by the Research Office of Hemp Genetics, Breeding and Comprehensive Utilization of Fujian Agriculture and Forestry University. The conference was held in Xiamen from June 19 to June 2 1 2008. Experts from China, the United States, Japan, South Korea, India, Malaysia, South Africa, Bangladesh, Indonesia, Uganda, Australia, Germany and other countries and regions attended the meeting. Dr. Sawar Kamal, Director-General of the United Nations International Jute Organization, Kazuhiko Tojima, Vice President of the Japanese Kenaf Association, Ke Yu Shao, Vice President of the Fujian Association for Science and Technology, and Major General Zhang Jianchun, Minister of the General Armament Department of the China People's Liberation Army, attended the meeting. On behalf of FAO, Dr. Sawar Kamal, Director-General of IHO, thanked Fujian Agriculture and Forestry University for its meticulous arrangement and hard work for the success of this international conference. He pointed out that this conference is another international exchange after the 2003 Beijing International Kenaf Conference, aiming at providing an effective platform for academic exchange, new technology development, international cooperation and project negotiation in the field of natural hemp fiber, and promoting the production, scientific research and industrialization of world hemp fiber.

2. Hold the National Hemp 948 Project and Modern Hemp Industry Seminar.

The National Hemp 948 Project and Modern Hemp Industry Seminar was held in Fuzhou on August 24-26, 2007. The conference was hosted by Fujian Agriculture and Forestry University, and more than 30 core experts from 948 cannabis project undertakers attended the conference. Dr. Liu Xu, vice president of China Academy of Agricultural Sciences, attended the meeting and made an important speech, affirming the achievements of the hemp industry and placing high hopes on the future industrial development; Xiong Heping, director of the Cannabis Research Institute of China Academy of Agricultural Sciences, made a special report on the research tasks and contents of the modern industrial technology system of cannabis. The person in charge of the hemp research office of Fujian Agriculture and Forestry University analyzed the bottleneck problems in the development of hemp industry, and put forward reasonable suggestions to solve the bottleneck problems. The delegates made a serious summary of the work of the 948 hemp project in the past year, which laid the foundation for the further development of hemp industry in China. 1, development ideas

(1) Carry out cross-disciplinary infiltration and horizontal cooperation, set up innovative research groups, jointly declare national key projects, improve the overall strength and level of hemp genetics and breeding, and strive to build the jute key functional laboratory of the Ministry of Agriculture in 2008.

(2) Take the national and provincial major scientific research plans as the research platform, organize cooperation in different fields, promote the integration of talents and technology, and improve the training level of master students and doctoral students.

(3) The combination of basic research and applied research, upstream and downstream research, modern biotechnology and traditional methods, genetic research and breeding research, comprehensive utilization and efficient transformation have developed a number of original achievements and solved major scientific, technical and production problems of cannabis science.

(4) With the aim of "advocating science, encouraging innovation, being pragmatic, seeking truth, making progress together and developing together", we will further strengthen the study style and the construction of the academic team of hemp genetics and breeding, and cultivate an academic team of hemp genetics and breeding that combines the old with the young, bears hardships and stands hard work, is united and dedicated, and is pioneering and innovative.

2. The main research objectives in the future:

(1) Further strengthen the research on the exploration, innovation and utilization of beneficial genes such as quality, disease resistance and stress resistance of hemp crop germplasm resources, clone beneficial genes such as crop quality, disease resistance, drought resistance, cold resistance and salt tolerance by using modern biotechnology, and provide genetic transformation and utilization;

(2) Further study on genetic map construction and gene mapping of cannabis and tobacco crops, and strengthen the research on the structure, function and expression regulation of cannabis and tobacco genome and protein group;

(3) Continue to study the origin, systematic evolution and classification of cannabis, as well as the core areas of molecular physiology and developmental biology of cannabis and tobacco;

(4) Continue to study the efficient and comprehensive utilization of hemp and castor by-products, focusing on the research and development of biomass conversion into bioenergy. 1, Construction of the First Kenaf Genetic Linkage Map in China: In 2007, the first Kenaf Genetic Linkage Map in China was preliminarily constructed using SRAP molecular markers. 143 marker loci entered 2 1 linkage group (LOD≥3.0), with a total length of 2267. 1cM. In 2008, a new drawing group was further used.

The genomic DNA of 203 individuals in f 2 mapping population was amplified by PCR, and the genetic linkage map of 208 polymorphic markers was constructed by Mapmaker/3.0. 143 markers entered 2 1 linkage group (LOD≥3.0), and the remaining 65 markers were independent, with a total length of 2267. 1cM, and each linkage group had 2-0 markers. The maximum spacing between markers was 49.2 cM, the minimum spacing was 0 cM, and the average spacing was 18.6cM. The distribution of markers in the whole linkage group was relatively uniform, and there was no aggregation of markers. This is the first genetic linkage map of kenaf constructed by SRAP, ISSR and RAPD markers.

2. Construction of tobacco genetic linkage map: On the basis of molecular markers of genetic diversity of tobacco germplasm resources, a mapping population of hybrid F2 between flue-cured tobacco and oriental tobacco was constructed in 2006. In 2007, ISSR and SRAP were used to amplify the mapping population, and a tobacco genetic linkage map consisting of 26 linkage groups and 1 12 marker loci was constructed, with a total length of 65438+. In 2008, this population was continuously marked by new markers, which is expected to build a tobacco genetic linkage map with high locus density and consistency.

3. Study on the drought tolerance of kenaf and the expression of protein group under drought stress: The changes of expression of protein group in kenaf under normal and drought conditions were studied. The specific proteins related to drought resistance were found by two-dimensional electrophoresis, and the peptide quality fingerprint was obtained by MALD-ToF mass spectrometry, which preliminarily clarified the mechanism of drought-tolerant varieties and the expression of functional proteins and regulatory proteins induced by drought stress.

4. protein Group Study on Cytoplasmic Male Sterility in Tobacco: Using Cytoplasmic Male Sterile Line and Maintainer Line, the differential expression of protein was determined at different developmental stages after the initial reproductive development, and the expression of specific proteins related to sterility was obtained by two-dimensional electrophoresis, and the information of specific proteins was obtained by MALD-ToF mass spectrometry. The specific proteins closely related to tobacco cytoplasmic male sterility protein were preliminarily identified, which laid a scientific foundation for revealing the molecular mechanism of tobacco male sterility.

5. Study on insect-resistant, herbicide-resistant and salt-tolerant transgenic kenaf: In 2007, insect-resistant transgenic kenaf Bt952 was successfully bred, which was proved to be stable for 6 generations by molecular hybridization. In the spring of 2007, the target gene of kenaf resistance was introduced in Hainan. The target genes of insect resistance, herbicide resistance and salt tolerance were detected from more than 3000 T0 generation materials, and further field identification and molecular hybridization were carried out in 2008.

6. Study on the transfer of cytoplasmic male sterile line of kenaf: In 2007, a new male sterile line with high combining ability was transferred from the introduced wild male sterile line A302 of kenaf, and the strong heterosis combination test was carried out. The stage of testing cross and transfer education has been completed.

7. Research on comprehensive utilization of kenaf by-products: In 2007, the research on enrichment of high linoleic acid and transformation of conjugated linoleic acid in kenaf was completed, and the research and development of new board, hemp plastic and hemp charcoal production technology with high oil absorption was completed, which laid a solid foundation for efficient utilization of kenaf biomass and research and development of new products.

8. Study on identification of favorable genes and innovative utilization of germplasm resources: Hundreds of kenaf germplasm resources were systematically identified for stress resistance, disease resistance and root-knot nematode resistance, and a number of excellent resources with multi-resistance, high yield and high quality were evaluated. With the introduction of foreign genes, space flight, mutation and transgenic technology, new kenaf germplasm with non-stinging stems, high linoleic acid content and insensitivity to light and new kenaf germplasm with insect resistance and herbicide resistance have been created. More than 400 kenaf, tobacco and castor germplasm resources were systematically classified by various molecular marker techniques, which provided scientific basis and technical platform for breeding and utilization.