Specifically, after adding DNA, synthetic raw materials (dNTP, primers) and thermostable DNA polymerase (taq) into EP tube, it will be denatured by heating, and the single strand will be copied into double strand under the action of polymerase, so as to achieve the purpose of gene replication.

Use:

1. Basic Research on Nucleic Acid: Genome Cloning

2. Asymmetric PCR is used to prepare single-stranded DNA for DNA sequencing.

3. The unknown DNA region was determined by reverse PCR.

4. Reverse transcription PCR(RT-PCR) is used to detect the level of gene expression in cells, the amount of RNA virus and directly clone the cDNA of specific genes.

5. Fluorescence quantitative PCR is used to monitor PCR products in real time.

6. Rapid amplification technology of 6.cDNA terminal

7. Detection of gene expression

8. Medical application: detection of bacterial and viral diseases; Diagnosing genetic diseases; Diagnose tumors; Applicable to court material evidence